Organelle Proteomics:

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purifi...

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Weitere Verfasser: Pflieger, Delphine (HerausgeberIn), Rossier, Jean (HerausgeberIn)
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: Totowa, NJ Humana Press 2008
Schriftenreihe:Methods in Molecular Biology™ 432
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Zusammenfassung:Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study
Beschreibung:1 Online-Ressource (XVII, 421 p)
ISBN:9781597450287
DOI:10.1007/978-1-59745-028-7

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