RNA detection: methods and protocols
This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mR...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Schriftenreihe: | Methods in Molecular Biology
1649 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs |
| Beschreibung: | 1 Online-Ressource (XIV, 492 Seiten) Illustrationen |
| ISBN: | 9781493972135 |
| DOI: | 10.1007/978-1-4939-7213-5 |
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| spelling | RNA detection methods and protocols edited by Imre Gaspar New York, NY Springer New York 2018 1 Online-Ressource (XIV, 492 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1649 This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs Biomedicine Human Genetics Medicine Human genetics RNS (DE-588)4076759-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS (DE-588)4076759-0 s Biochemische Analyse (DE-588)4255721-5 s Methode (DE-588)4038971-6 s 2\p DE-604 Gaspar, Imre (DE-588)1148462554 edt Erscheint auch als Druck-Ausgabe 978-1-4939-7212-8 https://doi.org/10.1007/978-1-4939-7213-5 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | RNA detection methods and protocols Biomedicine Human Genetics Medicine Human genetics RNS (DE-588)4076759-0 gnd Methode (DE-588)4038971-6 gnd Biochemische Analyse (DE-588)4255721-5 gnd |
| subject_GND | (DE-588)4076759-0 (DE-588)4038971-6 (DE-588)4255721-5 (DE-588)4143413-4 |
| title | RNA detection methods and protocols |
| title_auth | RNA detection methods and protocols |
| title_exact_search | RNA detection methods and protocols |
| title_full | RNA detection methods and protocols edited by Imre Gaspar |
| title_fullStr | RNA detection methods and protocols edited by Imre Gaspar |
| title_full_unstemmed | RNA detection methods and protocols edited by Imre Gaspar |
| title_short | RNA detection |
| title_sort | rna detection methods and protocols |
| title_sub | methods and protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics RNS (DE-588)4076759-0 gnd Methode (DE-588)4038971-6 gnd Biochemische Analyse (DE-588)4255721-5 gnd |
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