Shotgun Proteomics: Methods and Protocols

Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified, and characterized in a high throughput manner.  Beginning with the history of proteomics centered on the vital role of mass spectromet...

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Weitere Verfasser: Martins-de-Souza, Daniel (HerausgeberIn)
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: New York, NY Springer New York 2014
Schriftenreihe:Methods in Molecular Biology, Methods and Protocols 1156
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Zusammenfassung:Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified, and characterized in a high throughput manner.  Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics, and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species
Beschreibung:1 Online-Ressource (XIV, 492 p. 81 illus., 48 illus. in color)
ISBN:9781493906857
DOI:10.1007/978-1-4939-0685-7

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