Nuclease Methods and Protocols:
Nucleases, the enzymes that cleave and shape nucleic acids, are key regulators of cellular metabolism. Angiogenesis and the unfolded protein response are just a few instances of important intra- and extracellular pathways regulated by nucleases. In Nuclease Methods and Protocols, researchers at the...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in Molecular Biology™
160 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Nucleases, the enzymes that cleave and shape nucleic acids, are key regulators of cellular metabolism. Angiogenesis and the unfolded protein response are just a few instances of important intra- and extracellular pathways regulated by nucleases. In Nuclease Methods and Protocols, researchers at the forefront of academic research and pharmaceutical drug development from four continents summarize their recent results. Their projects involve nucleases in many different areas of medicine and biology, ranging from protein structure and folding, to DNA repair, to developing cures for insidious diseases such as cystic fibrosis, AIDS, and cancer. Each chapter contains a minireview of a specific nuclease or nuclease-related theme, a discussion of why and when to use the assays described, and often, an explanation of how the assay was developed. The authors describe their most valuable methods for determining the mode of action, structure, interaction with other molecules, and physiological role of nucleases. Detailed step-by-step instructions and notes on avoiding pitfalls help ensure readily reproducible results. Comprehensive and highly practical, Nuclease Methods and Protocols offers both novice and experienced researchers a deeper understanding of the importance of nucleases in cell metabolism, growth control mechanisms, and, increasingly, in diagnostics and therapeutics |
| Beschreibung: | 1 Online-Ressource (XVII, 525 p) |
| ISBN: | 9781592592333 |
| DOI: | 10.1385/1592592333 |
Internformat
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| 520 | |a Nucleases, the enzymes that cleave and shape nucleic acids, are key regulators of cellular metabolism. Angiogenesis and the unfolded protein response are just a few instances of important intra- and extracellular pathways regulated by nucleases. In Nuclease Methods and Protocols, researchers at the forefront of academic research and pharmaceutical drug development from four continents summarize their recent results. Their projects involve nucleases in many different areas of medicine and biology, ranging from protein structure and folding, to DNA repair, to developing cures for insidious diseases such as cystic fibrosis, AIDS, and cancer. Each chapter contains a minireview of a specific nuclease or nuclease-related theme, a discussion of why and when to use the assays described, and often, an explanation of how the assay was developed. The authors describe their most valuable methods for determining the mode of action, structure, interaction with other molecules, and physiological role of nucleases. Detailed step-by-step instructions and notes on avoiding pitfalls help ensure readily reproducible results. Comprehensive and highly practical, Nuclease Methods and Protocols offers both novice and experienced researchers a deeper understanding of the importance of nucleases in cell metabolism, growth control mechanisms, and, increasingly, in diagnostics and therapeutics | ||
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Datensatz im Suchindex
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| isbn | 9781592592333 |
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| spelling | Nuclease Methods and Protocols edited by Catherine H. Schein Totowa, NJ Humana Press 2001 1 Online-Ressource (XVII, 525 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 160 Nucleases, the enzymes that cleave and shape nucleic acids, are key regulators of cellular metabolism. Angiogenesis and the unfolded protein response are just a few instances of important intra- and extracellular pathways regulated by nucleases. In Nuclease Methods and Protocols, researchers at the forefront of academic research and pharmaceutical drug development from four continents summarize their recent results. Their projects involve nucleases in many different areas of medicine and biology, ranging from protein structure and folding, to DNA repair, to developing cures for insidious diseases such as cystic fibrosis, AIDS, and cancer. Each chapter contains a minireview of a specific nuclease or nuclease-related theme, a discussion of why and when to use the assays described, and often, an explanation of how the assay was developed. The authors describe their most valuable methods for determining the mode of action, structure, interaction with other molecules, and physiological role of nucleases. Detailed step-by-step instructions and notes on avoiding pitfalls help ensure readily reproducible results. Comprehensive and highly practical, Nuclease Methods and Protocols offers both novice and experienced researchers a deeper understanding of the importance of nucleases in cell metabolism, growth control mechanisms, and, increasingly, in diagnostics and therapeutics Life Sciences Cell Biology Life sciences Cell biology Nucleasen (DE-588)4172116-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Nucleasen (DE-588)4172116-0 s DE-604 Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s Schein, Catherine H. edt Erscheint auch als Druck-Ausgabe 9780896036796 https://doi.org/10.1385/1592592333 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Nuclease Methods and Protocols Life Sciences Cell Biology Life sciences Cell biology Nucleasen (DE-588)4172116-0 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4172116-0 (DE-588)4038971-6 (DE-588)4039983-7 (DE-588)4143413-4 |
| title | Nuclease Methods and Protocols |
| title_auth | Nuclease Methods and Protocols |
| title_exact_search | Nuclease Methods and Protocols |
| title_full | Nuclease Methods and Protocols edited by Catherine H. Schein |
| title_fullStr | Nuclease Methods and Protocols edited by Catherine H. Schein |
| title_full_unstemmed | Nuclease Methods and Protocols edited by Catherine H. Schein |
| title_short | Nuclease Methods and Protocols |
| title_sort | nuclease methods and protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Nucleasen (DE-588)4172116-0 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Nucleasen Methode Molekularbiologie Aufsatzsammlung |
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