Type 3 Secretion Systems: Methods and Protocols
This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its applica...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
|
| Schriftenreihe: | Methods in Molecular Biology
1531 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems |
| Beschreibung: | 1 Online-Ressource (X, 224 p. 32 illus., 23 illus. in color) |
| ISBN: | 9781493966493 |
| DOI: | 10.1007/978-1-4939-6649-3 |
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| spelling | Type 3 Secretion Systems Methods and Protocols edited by Matthew L. Nilles, Danielle L. Jessen Condry New York, NY Springer New York 2017 1 Online-Ressource (X, 224 p. 32 illus., 23 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1531 This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems Life Sciences Microbiology Life sciences Nilles, Matthew L. edt Condry, Danielle L. Jessen edt Erscheint auch als Druck-Ausgabe 9781493966479 https://doi.org/10.1007/978-1-4939-6649-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Type 3 Secretion Systems Methods and Protocols Life Sciences Microbiology Life sciences |
| title | Type 3 Secretion Systems Methods and Protocols |
| title_auth | Type 3 Secretion Systems Methods and Protocols |
| title_exact_search | Type 3 Secretion Systems Methods and Protocols |
| title_full | Type 3 Secretion Systems Methods and Protocols edited by Matthew L. Nilles, Danielle L. Jessen Condry |
| title_fullStr | Type 3 Secretion Systems Methods and Protocols edited by Matthew L. Nilles, Danielle L. Jessen Condry |
| title_full_unstemmed | Type 3 Secretion Systems Methods and Protocols edited by Matthew L. Nilles, Danielle L. Jessen Condry |
| title_short | Type 3 Secretion Systems |
| title_sort | type 3 secretion systems methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Microbiology Life sciences |
| topic_facet | Life Sciences Microbiology Life sciences |
| url | https://doi.org/10.1007/978-1-4939-6649-3 |
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