PCR Cloning Protocols: From Molecular Cloning to Genetic Engineering
The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. T...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1997
|
| Schriftenreihe: | Methods in Molecular Biology™
67 |
| Schlagworte: | |
| Online-Zugang: | UBR01 URL des Erstveröffentlichers |
| Zusammenfassung: | The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. This volume focuses on such preparative PCR protocols, which can be used in the cloning and modification of DNA. PCR Cloning Protocols: From Molecular Cloning to Genetic Engineer ing is divided into seven parts, each containing a collection of chapters address ing a general approach or goal. Part I presents basic PCR protocols, emphasizing optimizing conditions for (he amplification of DNA fragments of several kilobases in length. Part 11 offers several procedures for cloning PCR prod ucts, depending on whether a specific restriction site can be used in the clon ing vector, the PCR product is to be gel purified before cloning, or the fragmeni needs to be inserted in one or both orientations. Part III includes several pro tocols involved in the mutagenesis of DNA, either site-directed or not, as well as several approaches to recombinant PCR, either for mutagenesis or building a custom gene, as well as one chapter describing a specific use of in vitro selection. Part IV addresses the frequent need to amplify and clone segments of DNA that are to the right, left, or scattered within a stretch of DNA (either vector, chromosomal, or cDNA) of known sequence |
| Beschreibung: | 1 Online-Ressource (XIV, 490 p) |
| ISBN: | 9781592595532 |
| DOI: | 10.1385/0896034836 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044952316 | ||
| 003 | DE-604 | ||
| 005 | 20180911 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s1997 |||| o||u| ||||||eng d | ||
| 020 | |a 9781592595532 |9 978-1-59259-553-2 | ||
| 024 | 7 | |a 10.1385/0896034836 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59259-553-2 | ||
| 035 | |a (OCoLC)1036392545 | ||
| 035 | |a (DE-599)BVBBV044952316 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 | ||
| 050 | 0 | |a QH442.2 | |
| 082 | 0 | |a 574.87/3282 |2 20 | |
| 082 | 0 | |a 572 |2 23 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a WC 4400 |0 (DE-625)148099: |2 rvk | ||
| 084 | |a WC 4460 |0 (DE-625)148102: |2 rvk | ||
| 084 | |a WD 4150 |0 (DE-625)148177: |2 rvk | ||
| 084 | |a WG 1750 |0 (DE-625)148497: |2 rvk | ||
| 245 | 1 | 0 | |a PCR Cloning Protocols |b From Molecular Cloning to Genetic Engineering |c edited by Bruce A. White |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1997 | |
| 300 | |a 1 Online-Ressource (XIV, 490 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology™ |v 67 | |
| 520 | |a The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. This volume focuses on such preparative PCR protocols, which can be used in the cloning and modification of DNA. PCR Cloning Protocols: From Molecular Cloning to Genetic Engineer ing is divided into seven parts, each containing a collection of chapters address ing a general approach or goal. Part I presents basic PCR protocols, emphasizing optimizing conditions for (he amplification of DNA fragments of several kilobases in length. Part 11 offers several procedures for cloning PCR prod ucts, depending on whether a specific restriction site can be used in the clon ing vector, the PCR product is to be gel purified before cloning, or the fragmeni needs to be inserted in one or both orientations. Part III includes several pro tocols involved in the mutagenesis of DNA, either site-directed or not, as well as several approaches to recombinant PCR, either for mutagenesis or building a custom gene, as well as one chapter describing a specific use of in vitro selection. Part IV addresses the frequent need to amplify and clone segments of DNA that are to the right, left, or scattered within a stretch of DNA (either vector, chromosomal, or cDNA) of known sequence | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Biochemistry, general | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Biochemistry | |
| 650 | 0 | 7 | |a Genklonierung |0 (DE-588)4123275-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Genklonierung |0 (DE-588)4123275-6 |D s |
| 689 | 0 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |D s |
| 689 | 1 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 1 | |5 DE-604 | |
| 700 | 1 | |a White, Bruce A. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9780896034839 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/0896034836 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030345071 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1385/0896034836 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178542030749696 |
|---|---|
| any_adam_object | |
| author2 | White, Bruce A. |
| author2_role | edt |
| author2_variant | b a w ba baw |
| author_facet | White, Bruce A. |
| building | Verbundindex |
| bvnumber | BV044952316 |
| callnumber-first | Q - Science |
| callnumber-label | QH442 |
| callnumber-raw | QH442.2 |
| callnumber-search | QH442.2 |
| callnumber-sort | QH 3442.2 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 4150 WC 4400 WC 4460 WD 4150 WG 1750 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59259-553-2 (OCoLC)1036392545 (DE-599)BVBBV044952316 |
| dewey-full | 574.87/3282 572 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 574 - [Unassigned] 572 - Biochemistry |
| dewey-raw | 574.87/3282 572 |
| dewey-search | 574.87/3282 572 |
| dewey-sort | 3574.87 43282 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1385/0896034836 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03717nmm a2200625zcb4500</leader><controlfield tag="001">BV044952316</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180911 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s1997 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781592595532</subfield><subfield code="9">978-1-59259-553-2</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/0896034836</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59259-553-2</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1036392545</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044952316</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH442.2</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">574.87/3282</subfield><subfield code="2">20</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4400</subfield><subfield code="0">(DE-625)148099:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4460</subfield><subfield code="0">(DE-625)148102:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 4150</subfield><subfield code="0">(DE-625)148177:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WG 1750</subfield><subfield code="0">(DE-625)148497:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">PCR Cloning Protocols</subfield><subfield code="b">From Molecular Cloning to Genetic Engineering</subfield><subfield code="c">edited by Bruce A. White</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">1997</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XIV, 490 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology™</subfield><subfield code="v">67</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. This volume focuses on such preparative PCR protocols, which can be used in the cloning and modification of DNA. PCR Cloning Protocols: From Molecular Cloning to Genetic Engineer ing is divided into seven parts, each containing a collection of chapters address ing a general approach or goal. Part I presents basic PCR protocols, emphasizing optimizing conditions for (he amplification of DNA fragments of several kilobases in length. Part 11 offers several procedures for cloning PCR prod ucts, depending on whether a specific restriction site can be used in the clon ing vector, the PCR product is to be gel purified before cloning, or the fragmeni needs to be inserted in one or both orientations. Part III includes several pro tocols involved in the mutagenesis of DNA, either site-directed or not, as well as several approaches to recombinant PCR, either for mutagenesis or building a custom gene, as well as one chapter describing a specific use of in vitro selection. Part IV addresses the frequent need to amplify and clone segments of DNA that are to the right, left, or scattered within a stretch of DNA (either vector, chromosomal, or cDNA) of known sequence</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry, general</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Genklonierung</subfield><subfield code="0">(DE-588)4123275-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Polymerase-Kettenreaktion</subfield><subfield code="0">(DE-588)4256726-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Genklonierung</subfield><subfield code="0">(DE-588)4123275-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Polymerase-Kettenreaktion</subfield><subfield code="0">(DE-588)4256726-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">White, Bruce A.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9780896034839</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/0896034836</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030345071</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/0896034836</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV044952316 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:37Z |
| institution | BVB |
| isbn | 9781592595532 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030345071 |
| oclc_num | 1036392545 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 1 Online-Ressource (XIV, 490 p) |
| psigel | ZDB-2-PRO |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology™ |
| spelling | PCR Cloning Protocols From Molecular Cloning to Genetic Engineering edited by Bruce A. White Totowa, NJ Humana Press 1997 1 Online-Ressource (XIV, 490 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 67 The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization. However, creative investigators soon discovered the power of PCR for synthetic or preparative uses. This volume focuses on such preparative PCR protocols, which can be used in the cloning and modification of DNA. PCR Cloning Protocols: From Molecular Cloning to Genetic Engineer ing is divided into seven parts, each containing a collection of chapters address ing a general approach or goal. Part I presents basic PCR protocols, emphasizing optimizing conditions for (he amplification of DNA fragments of several kilobases in length. Part 11 offers several procedures for cloning PCR prod ucts, depending on whether a specific restriction site can be used in the clon ing vector, the PCR product is to be gel purified before cloning, or the fragmeni needs to be inserted in one or both orientations. Part III includes several pro tocols involved in the mutagenesis of DNA, either site-directed or not, as well as several approaches to recombinant PCR, either for mutagenesis or building a custom gene, as well as one chapter describing a specific use of in vitro selection. Part IV addresses the frequent need to amplify and clone segments of DNA that are to the right, left, or scattered within a stretch of DNA (either vector, chromosomal, or cDNA) of known sequence Life Sciences Biochemistry, general Life sciences Biochemistry Genklonierung (DE-588)4123275-6 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Genklonierung (DE-588)4123275-6 s Methode (DE-588)4038971-6 s DE-604 Polymerase-Kettenreaktion (DE-588)4256726-9 s White, Bruce A. edt Erscheint auch als Druck-Ausgabe 9780896034839 https://doi.org/10.1385/0896034836 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | PCR Cloning Protocols From Molecular Cloning to Genetic Engineering Life Sciences Biochemistry, general Life sciences Biochemistry Genklonierung (DE-588)4123275-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4123275-6 (DE-588)4256726-9 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | PCR Cloning Protocols From Molecular Cloning to Genetic Engineering |
| title_auth | PCR Cloning Protocols From Molecular Cloning to Genetic Engineering |
| title_exact_search | PCR Cloning Protocols From Molecular Cloning to Genetic Engineering |
| title_full | PCR Cloning Protocols From Molecular Cloning to Genetic Engineering edited by Bruce A. White |
| title_fullStr | PCR Cloning Protocols From Molecular Cloning to Genetic Engineering edited by Bruce A. White |
| title_full_unstemmed | PCR Cloning Protocols From Molecular Cloning to Genetic Engineering edited by Bruce A. White |
| title_short | PCR Cloning Protocols |
| title_sort | pcr cloning protocols from molecular cloning to genetic engineering |
| title_sub | From Molecular Cloning to Genetic Engineering |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Genklonierung (DE-588)4123275-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Genklonierung Polymerase-Kettenreaktion Methode Aufsatzsammlung |
| url | https://doi.org/10.1385/0896034836 |
| work_keys_str_mv | AT whitebrucea pcrcloningprotocolsfrommolecularcloningtogeneticengineering |