Protein-Ligand Interactions: Methods and Applications
Molecular recognition and binding of ligands (atoms, ions, and molecules) by proteins with high sensitivity and selectivity is of central importance to essentially all biomolecular processes and of key importance for the basic and applied sciences. In Protein-Ligand Interactions: Methods and Applica...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2005
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| Schriftenreihe: | Methods in Molecular Biology™
305 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Molecular recognition and binding of ligands (atoms, ions, and molecules) by proteins with high sensitivity and selectivity is of central importance to essentially all biomolecular processes and of key importance for the basic and applied sciences. In Protein-Ligand Interactions: Methods and Applications, leading experts with hands-on experience describe in detail a broad selection of established and emerging techniques for studying the interaction between proteins and ligands, including bulk biochemical techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved X-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Protein-Ligand Interactions: Methods and Applications offers novice and expert researchers alike a broad selection of powerful and widely applicable techniques that can be used to efficiently and successfully solve the task of characterizing protein-ligand interactions |
| Beschreibung: | 1 Online-Ressource (XI, 568 p) |
| ISBN: | 9781592599127 |
| DOI: | 10.1385/1592599125 |
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| spelling | Protein-Ligand Interactions Methods and Applications edited by G. Ulrich Nienhaus Totowa, NJ Humana Press 2005 1 Online-Ressource (XI, 568 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 305 Molecular recognition and binding of ligands (atoms, ions, and molecules) by proteins with high sensitivity and selectivity is of central importance to essentially all biomolecular processes and of key importance for the basic and applied sciences. In Protein-Ligand Interactions: Methods and Applications, leading experts with hands-on experience describe in detail a broad selection of established and emerging techniques for studying the interaction between proteins and ligands, including bulk biochemical techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved X-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Protein-Ligand Interactions: Methods and Applications offers novice and expert researchers alike a broad selection of powerful and widely applicable techniques that can be used to efficiently and successfully solve the task of characterizing protein-ligand interactions Life Sciences Biochemistry, general Life sciences Biochemistry Proteinbindung (DE-588)4047524-4 gnd rswk-swf Ligand Biochemie (DE-588)4606532-5 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Experiment (DE-588)4015999-1 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteinbindung (DE-588)4047524-4 s DE-604 Proteine (DE-588)4076388-2 s Ligand Biochemie (DE-588)4606532-5 s Wechselwirkung (DE-588)4064937-4 s Experiment (DE-588)4015999-1 s Nienhaus, Gerd Ulrich edt Erscheint auch als Druck-Ausgabe 9781588293725 https://doi.org/10.1385/1592599125 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Protein-Ligand Interactions Methods and Applications Life Sciences Biochemistry, general Life sciences Biochemistry Proteinbindung (DE-588)4047524-4 gnd Ligand Biochemie (DE-588)4606532-5 gnd Wechselwirkung (DE-588)4064937-4 gnd Experiment (DE-588)4015999-1 gnd Proteine (DE-588)4076388-2 gnd |
| subject_GND | (DE-588)4047524-4 (DE-588)4606532-5 (DE-588)4064937-4 (DE-588)4015999-1 (DE-588)4076388-2 (DE-588)4143413-4 |
| title | Protein-Ligand Interactions Methods and Applications |
| title_auth | Protein-Ligand Interactions Methods and Applications |
| title_exact_search | Protein-Ligand Interactions Methods and Applications |
| title_full | Protein-Ligand Interactions Methods and Applications edited by G. Ulrich Nienhaus |
| title_fullStr | Protein-Ligand Interactions Methods and Applications edited by G. Ulrich Nienhaus |
| title_full_unstemmed | Protein-Ligand Interactions Methods and Applications edited by G. Ulrich Nienhaus |
| title_short | Protein-Ligand Interactions |
| title_sort | protein ligand interactions methods and applications |
| title_sub | Methods and Applications |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Proteinbindung (DE-588)4047524-4 gnd Ligand Biochemie (DE-588)4606532-5 gnd Wechselwirkung (DE-588)4064937-4 gnd Experiment (DE-588)4015999-1 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Proteinbindung Ligand Biochemie Wechselwirkung Experiment Proteine Aufsatzsammlung |
| url | https://doi.org/10.1385/1592599125 |
| work_keys_str_mv | AT nienhausgerdulrich proteinligandinteractionsmethodsandapplications |