Protein Targeting Protocols:
In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods t...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1998
|
| Schriftenreihe: | Methods in Molecular Biology™
88 |
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it |
| Beschreibung: | 1 Online-Ressource (XII, 331 p) |
| ISBN: | 9781592595723 |
| DOI: | 10.1385/0896034879 |
Internformat
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| 520 | |a In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it | ||
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Datensatz im Suchindex
| _version_ | 1804178541892337664 |
|---|---|
| any_adam_object | |
| author2 | Clegg, Roger A. |
| author2_role | edt |
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| author_facet | Clegg, Roger A. |
| building | Verbundindex |
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| dewey-full | 572 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
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| discipline | Biologie |
| doi_str_mv | 10.1385/0896034879 |
| format | Electronic eBook |
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| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:37Z |
| institution | BVB |
| isbn | 9781592595723 |
| language | English |
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| physical | 1 Online-Ressource (XII, 331 p) |
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| publisher | Humana Press |
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| series2 | Methods in Molecular Biology™ |
| spelling | Protein Targeting Protocols edited by Roger A. Clegg Totowa, NJ Humana Press 1998 1 Online-Ressource (XII, 331 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 88 In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it Life Sciences Biochemistry, general Life sciences Biochemistry Proteinbindung (DE-588)4047524-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Proteintransport (DE-588)4273577-4 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Zielerkennung (DE-588)4190792-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteintransport (DE-588)4273577-4 s Zielerkennung (DE-588)4190792-9 s Methode (DE-588)4038971-6 s DE-604 Proteinbindung (DE-588)4047524-4 s Molekularbiologie (DE-588)4039983-7 s Clegg, Roger A. edt Erscheint auch als Druck-Ausgabe 9780896034877 https://doi.org/10.1385/0896034879 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Protein Targeting Protocols Life Sciences Biochemistry, general Life sciences Biochemistry Proteinbindung (DE-588)4047524-4 gnd Methode (DE-588)4038971-6 gnd Proteintransport (DE-588)4273577-4 gnd Molekularbiologie (DE-588)4039983-7 gnd Zielerkennung (DE-588)4190792-9 gnd |
| subject_GND | (DE-588)4047524-4 (DE-588)4038971-6 (DE-588)4273577-4 (DE-588)4039983-7 (DE-588)4190792-9 (DE-588)4143413-4 |
| title | Protein Targeting Protocols |
| title_auth | Protein Targeting Protocols |
| title_exact_search | Protein Targeting Protocols |
| title_full | Protein Targeting Protocols edited by Roger A. Clegg |
| title_fullStr | Protein Targeting Protocols edited by Roger A. Clegg |
| title_full_unstemmed | Protein Targeting Protocols edited by Roger A. Clegg |
| title_short | Protein Targeting Protocols |
| title_sort | protein targeting protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Proteinbindung (DE-588)4047524-4 gnd Methode (DE-588)4038971-6 gnd Proteintransport (DE-588)4273577-4 gnd Molekularbiologie (DE-588)4039983-7 gnd Zielerkennung (DE-588)4190792-9 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Proteinbindung Methode Proteintransport Molekularbiologie Zielerkennung Aufsatzsammlung |
| url | https://doi.org/10.1385/0896034879 |
| work_keys_str_mv | AT cleggrogera proteintargetingprotocols |