Telomeres and Telomerase: Methods and Protocols
The discovery of telomerase expression in a majority of tumors and the recognition of its potential role in the aging process has dramatically raised its profile as a target for therapeutic intervention. In Telomeres and Telomerase: Methods and Protocols, John A. Double and Michael J. Thompson have...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2002
|
| Schriftenreihe: | Methods in Molecular Biology™
191 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | The discovery of telomerase expression in a majority of tumors and the recognition of its potential role in the aging process has dramatically raised its profile as a target for therapeutic intervention. In Telomeres and Telomerase: Methods and Protocols, John A. Double and Michael J. Thompson have collected a critically important series of novel and essential techniques for studying telomeres and telomerase. These readily reproducible protocols provide cutting-edge tools to identify, measure, and analyze telomeres, to determine telomerase expression at the RNA level, to determine telomerase activity, and to detect potential modifiers of this activity. The techniques for assaying telomerase activity range from standard radiological TRAP assays to nonradioactive methods, from non-PCR-based methods to techniques using real-time PCR. Written by investigators who have used the methods extensively, each protocol includes step-by-step instructions, tips on avoiding pitfalls, and invaluable notes that make all the difference to successful experimental outcomes. Broad-ranging and highly practical, Telomeres and Telomerase: Methods and Protocols provides molecular and cell biologists, cancer researchers, and cellular pathologists with the core array of productive techniques needed today to develop telomerase inhibitors and clinically valuable diagnostic/prognostic telomerase markers |
| Beschreibung: | 1 Online-Ressource (XII, 224 p) |
| ISBN: | 9781592591893 |
| DOI: | 10.1385/1592591892 |
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Datensatz im Suchindex
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| spelling | Telomeres and Telomerase Methods and Protocols edited by John A. Double, Michael J. Thompson Totowa, NJ Humana Press 2002 1 Online-Ressource (XII, 224 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 191 The discovery of telomerase expression in a majority of tumors and the recognition of its potential role in the aging process has dramatically raised its profile as a target for therapeutic intervention. In Telomeres and Telomerase: Methods and Protocols, John A. Double and Michael J. Thompson have collected a critically important series of novel and essential techniques for studying telomeres and telomerase. These readily reproducible protocols provide cutting-edge tools to identify, measure, and analyze telomeres, to determine telomerase expression at the RNA level, to determine telomerase activity, and to detect potential modifiers of this activity. The techniques for assaying telomerase activity range from standard radiological TRAP assays to nonradioactive methods, from non-PCR-based methods to techniques using real-time PCR. Written by investigators who have used the methods extensively, each protocol includes step-by-step instructions, tips on avoiding pitfalls, and invaluable notes that make all the difference to successful experimental outcomes. Broad-ranging and highly practical, Telomeres and Telomerase: Methods and Protocols provides molecular and cell biologists, cancer researchers, and cellular pathologists with the core array of productive techniques needed today to develop telomerase inhibitors and clinically valuable diagnostic/prognostic telomerase markers Life Sciences Cell Biology Life sciences Cell biology Telomer Molekulargenetik (DE-588)4310952-4 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf Telomerase (DE-588)4513124-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Telomer Molekulargenetik (DE-588)4310952-4 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Telomerase (DE-588)4513124-7 s Double, John A. edt Thompson, Michael J. edt Erscheint auch als Druck-Ausgabe 9780896036574 https://doi.org/10.1385/1592591892 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Telomeres and Telomerase Methods and Protocols Life Sciences Cell Biology Life sciences Cell biology Telomer Molekulargenetik (DE-588)4310952-4 gnd Untersuchungsmethode (DE-588)4324907-3 gnd Telomerase (DE-588)4513124-7 gnd |
| subject_GND | (DE-588)4310952-4 (DE-588)4324907-3 (DE-588)4513124-7 (DE-588)4143413-4 |
| title | Telomeres and Telomerase Methods and Protocols |
| title_auth | Telomeres and Telomerase Methods and Protocols |
| title_exact_search | Telomeres and Telomerase Methods and Protocols |
| title_full | Telomeres and Telomerase Methods and Protocols edited by John A. Double, Michael J. Thompson |
| title_fullStr | Telomeres and Telomerase Methods and Protocols edited by John A. Double, Michael J. Thompson |
| title_full_unstemmed | Telomeres and Telomerase Methods and Protocols edited by John A. Double, Michael J. Thompson |
| title_short | Telomeres and Telomerase |
| title_sort | telomeres and telomerase methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Telomer Molekulargenetik (DE-588)4310952-4 gnd Untersuchungsmethode (DE-588)4324907-3 gnd Telomerase (DE-588)4513124-7 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Telomer Molekulargenetik Untersuchungsmethode Telomerase Aufsatzsammlung |
| url | https://doi.org/10.1385/1592591892 |
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