Cellular quiescence: methods and protocols
This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenera...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Schriftenreihe: | Methods in Molecular Biology
1686 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology |
| Beschreibung: | 1 Online-Ressource (XI, 303 Seiten) Illustrationen |
| ISBN: | 9781493973712 |
| DOI: | 10.1007/978-1-4939-7371-2 |
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| spelling | Cellular quiescence methods and protocols edited by H. Daniel Lacorazza New York, NY Springer New York 2018 1 Online-Ressource (XI, 303 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1686 This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology Life Sciences Cell Biology Life sciences Cell biology Lacorazza, H. Daniel (DE-588)1187963283 edt Erscheint auch als Druck-Ausgabe 9781493973705 https://doi.org/10.1007/978-1-4939-7371-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Cellular quiescence methods and protocols Life Sciences Cell Biology Life sciences Cell biology |
| title | Cellular quiescence methods and protocols |
| title_auth | Cellular quiescence methods and protocols |
| title_exact_search | Cellular quiescence methods and protocols |
| title_full | Cellular quiescence methods and protocols edited by H. Daniel Lacorazza |
| title_fullStr | Cellular quiescence methods and protocols edited by H. Daniel Lacorazza |
| title_full_unstemmed | Cellular quiescence methods and protocols edited by H. Daniel Lacorazza |
| title_short | Cellular quiescence |
| title_sort | cellular quiescence methods and protocols |
| title_sub | methods and protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology |
| url | https://doi.org/10.1007/978-1-4939-7371-2 |
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