Heterologous Gene Expression in E.coli: Methods and Protocols
This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screeni...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
|
| Schriftenreihe: | Methods in Molecular Biology
1586 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism |
| Beschreibung: | 1 Online-Ressource (XIV, 429 p. 92 illus., 55 illus. in color) |
| ISBN: | 9781493968879 |
| DOI: | 10.1007/978-1-4939-6887-9 |
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Datensatz im Suchindex
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| spelling | Heterologous Gene Expression in E.coli Methods and Protocols edited by Nicola A. Burgess-Brown New York, NY Springer New York 2017 1 Online-Ressource (XIV, 429 p. 92 illus., 55 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1586 This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism Life Sciences Microbiology Life sciences Burgess-Brown, Nicola A. edt Erscheint auch als Druck-Ausgabe 9781493968855 https://doi.org/10.1007/978-1-4939-6887-9 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Heterologous Gene Expression in E.coli Methods and Protocols Life Sciences Microbiology Life sciences |
| title | Heterologous Gene Expression in E.coli Methods and Protocols |
| title_auth | Heterologous Gene Expression in E.coli Methods and Protocols |
| title_exact_search | Heterologous Gene Expression in E.coli Methods and Protocols |
| title_full | Heterologous Gene Expression in E.coli Methods and Protocols edited by Nicola A. Burgess-Brown |
| title_fullStr | Heterologous Gene Expression in E.coli Methods and Protocols edited by Nicola A. Burgess-Brown |
| title_full_unstemmed | Heterologous Gene Expression in E.coli Methods and Protocols edited by Nicola A. Burgess-Brown |
| title_short | Heterologous Gene Expression in E.coli |
| title_sort | heterologous gene expression in e coli methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Microbiology Life sciences |
| topic_facet | Life Sciences Microbiology Life sciences |
| url | https://doi.org/10.1007/978-1-4939-6887-9 |
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