Synthetic Antibodies: Methods and Protocols
This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic ant...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
|
| Schriftenreihe: | Methods in Molecular Biology
1575 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research |
| Beschreibung: | 1 Online-Ressource (XIV, 420 p. 102 illus., 74 illus. in color) |
| ISBN: | 9781493968572 |
| DOI: | 10.1007/978-1-4939-6857-2 |
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| spelling | Synthetic Antibodies Methods and Protocols edited by Thomas Tiller New York, NY Springer New York 2017 1 Online-Ressource (XIV, 420 p. 102 illus., 74 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1575 This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research Biomedicine Immunology Medicine Tiller, Thomas edt Erscheint auch als Druck-Ausgabe 9781493968558 https://doi.org/10.1007/978-1-4939-6857-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Synthetic Antibodies Methods and Protocols Biomedicine Immunology Medicine |
| title | Synthetic Antibodies Methods and Protocols |
| title_auth | Synthetic Antibodies Methods and Protocols |
| title_exact_search | Synthetic Antibodies Methods and Protocols |
| title_full | Synthetic Antibodies Methods and Protocols edited by Thomas Tiller |
| title_fullStr | Synthetic Antibodies Methods and Protocols edited by Thomas Tiller |
| title_full_unstemmed | Synthetic Antibodies Methods and Protocols edited by Thomas Tiller |
| title_short | Synthetic Antibodies |
| title_sort | synthetic antibodies methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Immunology Medicine |
| topic_facet | Biomedicine Immunology Medicine |
| url | https://doi.org/10.1007/978-1-4939-6857-2 |
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