The BAM Complex: Methods and Protocols

This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex...

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Bibliographische Detailangaben
Weitere Verfasser: Buchanan, Susan (HerausgeberIn), Noinaj, Nicholas (HerausgeberIn)
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: New York, NY Springer New York 2015
Ausgabe:1st ed. 2015
Schriftenreihe:Methods in Molecular Biology 1329
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Zusammenfassung:This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex
Beschreibung:1 Online-Ressource (XI, 283 p. 43 illus., 16 illus. in color)
ISBN:9781493928712
DOI:10.1007/978-1-4939-2871-2

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