Baculovirus and Insect Cell Expression Protocols:
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2016
|
| Ausgabe: | 3rd ed. 2016 |
| Schriftenreihe: | Methods in Molecular Biology
1350 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture |
| Beschreibung: | 1 Online-Ressource (XII, 508 p. 65 illus., 15 illus. in color) |
| ISBN: | 9781493930432 |
| DOI: | 10.1007/978-1-4939-3043-2 |
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Datensatz im Suchindex
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| any_adam_object | |
| author2 | Murhammer, David W. |
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| dewey-full | 579 579.2436 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 579 - Microorganisms, fungi & algae |
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| dewey-search | 579 579.2436 |
| dewey-sort | 3579 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-4939-3043-2 |
| edition | 3rd ed. 2016 |
| format | Electronic eBook |
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| title | Baculovirus and Insect Cell Expression Protocols |
| title_auth | Baculovirus and Insect Cell Expression Protocols |
| title_exact_search | Baculovirus and Insect Cell Expression Protocols |
| title_full | Baculovirus and Insect Cell Expression Protocols edited by David W. Murhammer |
| title_fullStr | Baculovirus and Insect Cell Expression Protocols edited by David W. Murhammer |
| title_full_unstemmed | Baculovirus and Insect Cell Expression Protocols edited by David W. Murhammer |
| title_short | Baculovirus and Insect Cell Expression Protocols |
| title_sort | baculovirus and insect cell expression protocols |
| topic | Life Sciences Microbiology Virology Life sciences Baculoviren (DE-588)4130674-0 gnd Virusvektor (DE-588)4232137-2 gnd Gentechnologie (DE-588)4071722-7 gnd Insektenviren (DE-588)4193789-2 gnd Genexpression (DE-588)4020136-3 gnd Insekten (DE-588)4027110-9 gnd Vektor Genetik (DE-588)4279246-0 gnd |
| topic_facet | Life Sciences Microbiology Virology Life sciences Baculoviren Virusvektor Gentechnologie Insektenviren Genexpression Insekten Vektor Genetik Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-3043-2 |
| work_keys_str_mv | AT murhammerdavidw baculovirusandinsectcellexpressionprotocols |