Neurotrophic factors: methods and protocols
This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral n...
Gespeichert in:
Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
New York, NY
Springer New York
2018
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Ausgabe: | 2nd ed. 2018 |
Schriftenreihe: | Methods in Molecular Biology
1727 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology |
Beschreibung: | 1 Online-Ressource (XV, 458 Seiten) Illustrationen |
ISBN: | 9781493975716 |
DOI: | 10.1007/978-1-4939-7571-6 |
Internformat
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Datensatz im Suchindex
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dewey-full | 612.8 |
dewey-hundreds | 600 - Technology (Applied sciences) |
dewey-ones | 612 - Human physiology |
dewey-raw | 612.8 |
dewey-search | 612.8 |
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discipline | Medizin |
doi_str_mv | 10.1007/978-1-4939-7571-6 |
edition | 2nd ed. 2018 |
format | Electronic eBook |
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spelling | Neurotrophic factors methods and protocols edited by Stephen D. Skaper 2nd ed. 2018 New York, NY Springer New York 2018 1 Online-Ressource (XV, 458 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1727 This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology Biomedicine Neurosciences Medicine Neurotropher Faktor (DE-588)4317638-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Neurotropher Faktor (DE-588)4317638-0 s Methode (DE-588)4038971-6 s 2\p DE-604 Skaper, Stephen D. (DE-588)1152081837 edt Erscheint auch als Druck-Ausgabe 9781493975709 https://doi.org/10.1007/978-1-4939-7571-6 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Neurotrophic factors methods and protocols Biomedicine Neurosciences Medicine Neurotropher Faktor (DE-588)4317638-0 gnd Methode (DE-588)4038971-6 gnd |
subject_GND | (DE-588)4317638-0 (DE-588)4038971-6 (DE-588)4143413-4 |
title | Neurotrophic factors methods and protocols |
title_auth | Neurotrophic factors methods and protocols |
title_exact_search | Neurotrophic factors methods and protocols |
title_full | Neurotrophic factors methods and protocols edited by Stephen D. Skaper |
title_fullStr | Neurotrophic factors methods and protocols edited by Stephen D. Skaper |
title_full_unstemmed | Neurotrophic factors methods and protocols edited by Stephen D. Skaper |
title_short | Neurotrophic factors |
title_sort | neurotrophic factors methods and protocols |
title_sub | methods and protocols |
topic | Biomedicine Neurosciences Medicine Neurotropher Faktor (DE-588)4317638-0 gnd Methode (DE-588)4038971-6 gnd |
topic_facet | Biomedicine Neurosciences Medicine Neurotropher Faktor Methode Aufsatzsammlung |
url | https://doi.org/10.1007/978-1-4939-7571-6 |
work_keys_str_mv | AT skaperstephend neurotrophicfactorsmethodsandprotocols |