Gene Knockout Protocols: Second Edition
With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In Gene K...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2009
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
530 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In Gene Knockout Protocols, Second Edition, distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory protocols. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Gene Knockout Protocols, Second Edition provides a unique collection of expert bench protocols certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today |
| Beschreibung: | 1 Online-Ressource (XVI, 516 p) |
| ISBN: | 9781597454711 |
| DOI: | 10.1007/978-1-59745-471-1 |
Internformat
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| spelling | Gene Knockout Protocols Second Edition edited by Wolfgang Wurst, Ralf Kühn Totowa, NJ Humana Press 2009 1 Online-Ressource (XVI, 516 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 530 With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In Gene Knockout Protocols, Second Edition, distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory protocols. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Gene Knockout Protocols, Second Edition provides a unique collection of expert bench protocols certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today Life Sciences Biochemistry, general Human Genetics Animal Genetics and Genomics Animal Models Life sciences Human genetics Biochemistry Animal genetics Animal models in research Gen (DE-588)4128987-0 gnd rswk-swf Gen-Targeting (DE-588)4294998-1 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Mutante (DE-588)4256709-9 gnd rswk-swf Transgene Tiere (DE-588)4279021-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Maus (DE-588)4169148-9 s Mutante (DE-588)4256709-9 s Gen (DE-588)4128987-0 s DE-604 Transgene Tiere (DE-588)4279021-9 s Gen-Targeting (DE-588)4294998-1 s Wurst, Wolfgang edt Kühn, Ralf edt Erscheint auch als Druck-Ausgabe 9781934115268 https://doi.org/10.1007/978-1-59745-471-1 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Gene Knockout Protocols Second Edition Life Sciences Biochemistry, general Human Genetics Animal Genetics and Genomics Animal Models Life sciences Human genetics Biochemistry Animal genetics Animal models in research Gen (DE-588)4128987-0 gnd Gen-Targeting (DE-588)4294998-1 gnd Maus (DE-588)4169148-9 gnd Mutante (DE-588)4256709-9 gnd Transgene Tiere (DE-588)4279021-9 gnd |
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| title | Gene Knockout Protocols Second Edition |
| title_auth | Gene Knockout Protocols Second Edition |
| title_exact_search | Gene Knockout Protocols Second Edition |
| title_full | Gene Knockout Protocols Second Edition edited by Wolfgang Wurst, Ralf Kühn |
| title_fullStr | Gene Knockout Protocols Second Edition edited by Wolfgang Wurst, Ralf Kühn |
| title_full_unstemmed | Gene Knockout Protocols Second Edition edited by Wolfgang Wurst, Ralf Kühn |
| title_short | Gene Knockout Protocols |
| title_sort | gene knockout protocols second edition |
| title_sub | Second Edition |
| topic | Life Sciences Biochemistry, general Human Genetics Animal Genetics and Genomics Animal Models Life sciences Human genetics Biochemistry Animal genetics Animal models in research Gen (DE-588)4128987-0 gnd Gen-Targeting (DE-588)4294998-1 gnd Maus (DE-588)4169148-9 gnd Mutante (DE-588)4256709-9 gnd Transgene Tiere (DE-588)4279021-9 gnd |
| topic_facet | Life Sciences Biochemistry, general Human Genetics Animal Genetics and Genomics Animal Models Life sciences Human genetics Biochemistry Animal genetics Animal models in research Gen Gen-Targeting Maus Mutante Transgene Tiere Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-59745-471-1 |
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