Crystallographic Methods and Protocols:
The volumes in the series, Methods in Molecular Biology, are conceived with the biochemist and molecular biologist in mind. The present book, Crystallographic Methods andProtocols, concentrates on the use of X-ray crystallography to solve the detailed three dimensional structuresofproteins, nucleic...
Gespeichert in:
| Weitere Verfasser: | , , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1996
|
| Schriftenreihe: | Methods in Molecular Biology™
56 |
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | The volumes in the series, Methods in Molecular Biology, are conceived with the biochemist and molecular biologist in mind. The present book, Crystallographic Methods andProtocols, concentrates on the use of X-ray crystallography to solve the detailed three dimensional structuresofproteins, nucleic acids, andtheir complexes. Such a structure determination is a major undertaking, demands expertise in a range of skills, and requires considerable resources. The biologically trained worker will probably first become involved when identifying an important scientific problem whose solution would benefit from a full structure. The protein or nucleic acid at issue must be sequenced and prepared to high purity in appropriate quantities, probably by either chemical synthesis for nucleic acids or genetic engineering for proteins. CrystallographicMethods andPro tocols aims to give biologically trained workers an insight into the techniques used to crystallize their proteins, obtain the raw X-ray data, and solve and refine the structure. The aim ofa crystal structure determination is to provide infor mation that will solve biologically relevant problems; that process normally requires a high resolution structure. The preparationofsuit able crystals for a high resolution structure remains the major bottle neck in structure determination, and the effective application of appropriate genetic engineering and biochemical techniques in the initial stages pays ahandsome dividend later. The productionofsuit able proteins by recombinant methods and the preparationofcrystal line derivatives are covered by Skelly and Madden in Chapter 2. Before beginning the data collection required for a high resolution structure, a preliminary characterizationofthe crystals is carried out |
| Beschreibung: | 1 Online-Ressource (408 p) |
| ISBN: | 9781592595433 |
| DOI: | 10.1385/0896032590 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044952089 | ||
| 003 | DE-604 | ||
| 005 | 20180911 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s1996 |||| o||u| ||||||eng d | ||
| 020 | |a 9781592595433 |9 978-1-59259-543-3 | ||
| 024 | 7 | |a 10.1385/0896032590 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59259-543-3 | ||
| 035 | |a (OCoLC)1036376181 | ||
| 035 | |a (DE-599)BVBBV044952089 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 | ||
| 082 | 0 | |a 543 |2 23 | |
| 084 | |a WC 2700 |0 (DE-625)148072: |2 rvk | ||
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a WC 4170 |0 (DE-625)148093: |2 rvk | ||
| 084 | |a BIO 220f |2 stub | ||
| 084 | |a CHE 806f |2 stub | ||
| 084 | |a CHE 810f |2 stub | ||
| 084 | |a BIO 040f |2 stub | ||
| 245 | 1 | 0 | |a Crystallographic Methods and Protocols |c edited by Christopher Jones, Barbara Mulloy, Mark R. Sanderson |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1996 | |
| 300 | |a 1 Online-Ressource (408 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology™ |v 56 | |
| 520 | |a The volumes in the series, Methods in Molecular Biology, are conceived with the biochemist and molecular biologist in mind. The present book, Crystallographic Methods andProtocols, concentrates on the use of X-ray crystallography to solve the detailed three dimensional structuresofproteins, nucleic acids, andtheir complexes. Such a structure determination is a major undertaking, demands expertise in a range of skills, and requires considerable resources. The biologically trained worker will probably first become involved when identifying an important scientific problem whose solution would benefit from a full structure. The protein or nucleic acid at issue must be sequenced and prepared to high purity in appropriate quantities, probably by either chemical synthesis for nucleic acids or genetic engineering for proteins. CrystallographicMethods andPro tocols aims to give biologically trained workers an insight into the techniques used to crystallize their proteins, obtain the raw X-ray data, and solve and refine the structure. The aim ofa crystal structure determination is to provide infor mation that will solve biologically relevant problems; that process normally requires a high resolution structure. The preparationofsuit able crystals for a high resolution structure remains the major bottle neck in structure determination, and the effective application of appropriate genetic engineering and biochemical techniques in the initial stages pays ahandsome dividend later. The productionofsuit able proteins by recombinant methods and the preparationofcrystal line derivatives are covered by Skelly and Madden in Chapter 2. Before beginning the data collection required for a high resolution structure, a preliminary characterizationofthe crystals is carried out | ||
| 650 | 4 | |a Chemistry | |
| 650 | 4 | |a Analytical Chemistry | |
| 650 | 4 | |a Chemistry | |
| 650 | 4 | |a Analytical chemistry | |
| 650 | 0 | 7 | |a Molekularbiologie |0 (DE-588)4039983-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Kristallographie |0 (DE-588)4033217-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Kristallographie |0 (DE-588)4033217-2 |D s |
| 689 | 0 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Molekularbiologie |0 (DE-588)4039983-7 |D s |
| 689 | 1 | 1 | |a Kristallographie |0 (DE-588)4033217-2 |D s |
| 689 | 1 | 2 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 1 | |5 DE-604 | |
| 700 | 1 | |a Jones, Christopher |4 edt | |
| 700 | 1 | |a Mulloy, Barbara |4 edt | |
| 700 | 1 | |a Sanderson, Mark R. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9780896032590 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/0896032590 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030344845 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1385/0896032590 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178541543161856 |
|---|---|
| any_adam_object | |
| author2 | Jones, Christopher Mulloy, Barbara Sanderson, Mark R. |
| author2_role | edt edt edt |
| author2_variant | c j cj b m bm m r s mr mrs |
| author_facet | Jones, Christopher Mulloy, Barbara Sanderson, Mark R. |
| building | Verbundindex |
| bvnumber | BV044952089 |
| classification_rvk | WC 2700 WC 4150 WC 4170 |
| classification_tum | BIO 220f CHE 806f CHE 810f BIO 040f |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59259-543-3 (OCoLC)1036376181 (DE-599)BVBBV044952089 |
| dewey-full | 543 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 543 - Analytical chemistry |
| dewey-raw | 543 |
| dewey-search | 543 |
| dewey-sort | 3543 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Biologie Chemie |
| doi_str_mv | 10.1385/0896032590 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>04136nmm a2200661zcb4500</leader><controlfield tag="001">BV044952089</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180911 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s1996 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781592595433</subfield><subfield code="9">978-1-59259-543-3</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/0896032590</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59259-543-3</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1036376181</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044952089</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">543</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 2700</subfield><subfield code="0">(DE-625)148072:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4170</subfield><subfield code="0">(DE-625)148093:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIO 220f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 806f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 810f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIO 040f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Crystallographic Methods and Protocols</subfield><subfield code="c">edited by Christopher Jones, Barbara Mulloy, Mark R. Sanderson</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">1996</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (408 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology™</subfield><subfield code="v">56</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The volumes in the series, Methods in Molecular Biology, are conceived with the biochemist and molecular biologist in mind. The present book, Crystallographic Methods andProtocols, concentrates on the use of X-ray crystallography to solve the detailed three dimensional structuresofproteins, nucleic acids, andtheir complexes. Such a structure determination is a major undertaking, demands expertise in a range of skills, and requires considerable resources. The biologically trained worker will probably first become involved when identifying an important scientific problem whose solution would benefit from a full structure. The protein or nucleic acid at issue must be sequenced and prepared to high purity in appropriate quantities, probably by either chemical synthesis for nucleic acids or genetic engineering for proteins. CrystallographicMethods andPro tocols aims to give biologically trained workers an insight into the techniques used to crystallize their proteins, obtain the raw X-ray data, and solve and refine the structure. The aim ofa crystal structure determination is to provide infor mation that will solve biologically relevant problems; that process normally requires a high resolution structure. The preparationofsuit able crystals for a high resolution structure remains the major bottle neck in structure determination, and the effective application of appropriate genetic engineering and biochemical techniques in the initial stages pays ahandsome dividend later. The productionofsuit able proteins by recombinant methods and the preparationofcrystal line derivatives are covered by Skelly and Madden in Chapter 2. Before beginning the data collection required for a high resolution structure, a preliminary characterizationofthe crystals is carried out</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Analytical Chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Analytical chemistry</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Molekularbiologie</subfield><subfield code="0">(DE-588)4039983-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Kristallographie</subfield><subfield code="0">(DE-588)4033217-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Kristallographie</subfield><subfield code="0">(DE-588)4033217-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Molekularbiologie</subfield><subfield code="0">(DE-588)4039983-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Kristallographie</subfield><subfield code="0">(DE-588)4033217-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="2"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jones, Christopher</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mulloy, Barbara</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sanderson, Mark R.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9780896032590</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/0896032590</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030344845</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/0896032590</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV044952089 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:37Z |
| institution | BVB |
| isbn | 9781592595433 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030344845 |
| oclc_num | 1036376181 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 1 Online-Ressource (408 p) |
| psigel | ZDB-2-PRO |
| publishDate | 1996 |
| publishDateSearch | 1996 |
| publishDateSort | 1996 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology™ |
| spelling | Crystallographic Methods and Protocols edited by Christopher Jones, Barbara Mulloy, Mark R. Sanderson Totowa, NJ Humana Press 1996 1 Online-Ressource (408 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 56 The volumes in the series, Methods in Molecular Biology, are conceived with the biochemist and molecular biologist in mind. The present book, Crystallographic Methods andProtocols, concentrates on the use of X-ray crystallography to solve the detailed three dimensional structuresofproteins, nucleic acids, andtheir complexes. Such a structure determination is a major undertaking, demands expertise in a range of skills, and requires considerable resources. The biologically trained worker will probably first become involved when identifying an important scientific problem whose solution would benefit from a full structure. The protein or nucleic acid at issue must be sequenced and prepared to high purity in appropriate quantities, probably by either chemical synthesis for nucleic acids or genetic engineering for proteins. CrystallographicMethods andPro tocols aims to give biologically trained workers an insight into the techniques used to crystallize their proteins, obtain the raw X-ray data, and solve and refine the structure. The aim ofa crystal structure determination is to provide infor mation that will solve biologically relevant problems; that process normally requires a high resolution structure. The preparationofsuit able crystals for a high resolution structure remains the major bottle neck in structure determination, and the effective application of appropriate genetic engineering and biochemical techniques in the initial stages pays ahandsome dividend later. The productionofsuit able proteins by recombinant methods and the preparationofcrystal line derivatives are covered by Skelly and Madden in Chapter 2. Before beginning the data collection required for a high resolution structure, a preliminary characterizationofthe crystals is carried out Chemistry Analytical Chemistry Analytical chemistry Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Kristallographie (DE-588)4033217-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Kristallographie (DE-588)4033217-2 s Methode (DE-588)4038971-6 s DE-604 Molekularbiologie (DE-588)4039983-7 s Jones, Christopher edt Mulloy, Barbara edt Sanderson, Mark R. edt Erscheint auch als Druck-Ausgabe 9780896032590 https://doi.org/10.1385/0896032590 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Crystallographic Methods and Protocols Chemistry Analytical Chemistry Analytical chemistry Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Kristallographie (DE-588)4033217-2 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4038971-6 (DE-588)4033217-2 (DE-588)4143413-4 |
| title | Crystallographic Methods and Protocols |
| title_auth | Crystallographic Methods and Protocols |
| title_exact_search | Crystallographic Methods and Protocols |
| title_full | Crystallographic Methods and Protocols edited by Christopher Jones, Barbara Mulloy, Mark R. Sanderson |
| title_fullStr | Crystallographic Methods and Protocols edited by Christopher Jones, Barbara Mulloy, Mark R. Sanderson |
| title_full_unstemmed | Crystallographic Methods and Protocols edited by Christopher Jones, Barbara Mulloy, Mark R. Sanderson |
| title_short | Crystallographic Methods and Protocols |
| title_sort | crystallographic methods and protocols |
| topic | Chemistry Analytical Chemistry Analytical chemistry Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Kristallographie (DE-588)4033217-2 gnd |
| topic_facet | Chemistry Analytical Chemistry Analytical chemistry Molekularbiologie Methode Kristallographie Aufsatzsammlung |
| url | https://doi.org/10.1385/0896032590 |
| work_keys_str_mv | AT joneschristopher crystallographicmethodsandprotocols AT mulloybarbara crystallographicmethodsandprotocols AT sandersonmarkr crystallographicmethodsandprotocols |