Gene Expression Profiling: Methods and Protocols
The transcription of messenger RNA from a DNA template is a key process in a wide variety of biological systems. In Gene Expression Profiling: Methods and Protocols, leaders in gene expression methodology and bioinformatics data analysis share their best methods for measuring RNA levels in cells and...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
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| Schriftenreihe: | Methods in Molecular Biology
258 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | The transcription of messenger RNA from a DNA template is a key process in a wide variety of biological systems. In Gene Expression Profiling: Methods and Protocols, leaders in gene expression methodology and bioinformatics data analysis share their best methods for measuring RNA levels in cells and tissues. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Special care has been taken to distill the basic underlying principles of each method to a few straightforward concepts so that investigators can easily choose the method most appropriate to their application. The readily reproducible techniques presented include new methods for applying the GeneChip, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. Cutting-edge and highly practical, Gene Expression Profiling: Methods and Protocols allows novice and experienced investigators alike not only to choose the best method to determine how genes are turned on and off in many different organisms, but also to use informatics resources to analyze and understand the results |
| Beschreibung: | 1 Online-Ressource (X, 168 p) |
| ISBN: | 9781592597512 |
| DOI: | 10.1385/1592597513 |
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| spelling | Gene Expression Profiling Methods and Protocols edited by Richard A. Shimkets Totowa, NJ Humana Press 2004 1 Online-Ressource (X, 168 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 258 The transcription of messenger RNA from a DNA template is a key process in a wide variety of biological systems. In Gene Expression Profiling: Methods and Protocols, leaders in gene expression methodology and bioinformatics data analysis share their best methods for measuring RNA levels in cells and tissues. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Special care has been taken to distill the basic underlying principles of each method to a few straightforward concepts so that investigators can easily choose the method most appropriate to their application. The readily reproducible techniques presented include new methods for applying the GeneChip, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. Cutting-edge and highly practical, Gene Expression Profiling: Methods and Protocols allows novice and experienced investigators alike not only to choose the best method to determine how genes are turned on and off in many different organisms, but also to use informatics resources to analyze and understand the results Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Genexpression (DE-588)4020136-3 s Labortechnik (DE-588)4123602-6 s b DE-604 Shimkets, Richard A. edt Erscheint auch als Druck-Ausgabe 9781588292209 https://doi.org/10.1385/1592597513 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Gene Expression Profiling Methods and Protocols Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4020136-3 (DE-588)4143413-4 |
| title | Gene Expression Profiling Methods and Protocols |
| title_auth | Gene Expression Profiling Methods and Protocols |
| title_exact_search | Gene Expression Profiling Methods and Protocols |
| title_full | Gene Expression Profiling Methods and Protocols edited by Richard A. Shimkets |
| title_fullStr | Gene Expression Profiling Methods and Protocols edited by Richard A. Shimkets |
| title_full_unstemmed | Gene Expression Profiling Methods and Protocols edited by Richard A. Shimkets |
| title_short | Gene Expression Profiling |
| title_sort | gene expression profiling methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Labortechnik Genexpression Aufsatzsammlung |
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