Heterologous Protein Production in CHO Cells: Methods and Protocols
This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
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| Schriftenreihe: | Methods in Molecular Biology
1603 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells |
| Beschreibung: | 1 Online-Ressource (X, 252 p. 35 illus., 28 illus. in color) |
| ISBN: | 9781493969722 |
| DOI: | 10.1007/978-1-4939-6972-2 |
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| spelling | Heterologous Protein Production in CHO Cells Methods and Protocols edited by Paula Meleady New York, NY Springer New York 2017 1 Online-Ressource (X, 252 p. 35 illus., 28 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1603 This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells Life Sciences Protein Science Life sciences Proteins Meleady, Paula edt Erscheint auch als Druck-Ausgabe 9781493969715 https://doi.org/10.1007/978-1-4939-6972-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Heterologous Protein Production in CHO Cells Methods and Protocols Life Sciences Protein Science Life sciences Proteins |
| title | Heterologous Protein Production in CHO Cells Methods and Protocols |
| title_auth | Heterologous Protein Production in CHO Cells Methods and Protocols |
| title_exact_search | Heterologous Protein Production in CHO Cells Methods and Protocols |
| title_full | Heterologous Protein Production in CHO Cells Methods and Protocols edited by Paula Meleady |
| title_fullStr | Heterologous Protein Production in CHO Cells Methods and Protocols edited by Paula Meleady |
| title_full_unstemmed | Heterologous Protein Production in CHO Cells Methods and Protocols edited by Paula Meleady |
| title_short | Heterologous Protein Production in CHO Cells |
| title_sort | heterologous protein production in cho cells methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Protein Science Life sciences Proteins |
| topic_facet | Life Sciences Protein Science Life sciences Proteins |
| url | https://doi.org/10.1007/978-1-4939-6972-2 |
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