Cell-Free Protein Synthesis: Methods and Protocols
Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic inform...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2014
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1118 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies. |
| Beschreibung: | 1 Online-Ressource (XI, 313 p. 66 illus., 31 illus. in color) |
| ISBN: | 9781627037822 |
| DOI: | 10.1007/978-1-62703-782-2 |
Internformat
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| 520 | |a Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies. | ||
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Datensatz im Suchindex
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| author2 | Alexandrov, Kirill Johnston, Wayne A. |
| author2_role | edt edt |
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| building | Verbundindex |
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| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-62703-782-2 |
| format | Electronic eBook |
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| spelling | Cell-Free Protein Synthesis Methods and Protocols edited by Kirill Alexandrov, Wayne A. Johnston Totowa, NJ Humana Press 2014 1 Online-Ressource (XI, 313 p. 66 illus., 31 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1118 Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies. Life Sciences Protein Science Life sciences Proteins Zellfreies System (DE-588)4342432-6 gnd rswk-swf Proteinbiosynthese (DE-588)4175987-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteinbiosynthese (DE-588)4175987-4 s Zellfreies System (DE-588)4342432-6 s Methode (DE-588)4038971-6 s b DE-604 Alexandrov, Kirill edt Johnston, Wayne A. edt Erscheint auch als Druck-Ausgabe 9781627037815 https://doi.org/10.1007/978-1-62703-782-2 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Cell-Free Protein Synthesis Methods and Protocols Life Sciences Protein Science Life sciences Proteins Zellfreies System (DE-588)4342432-6 gnd Proteinbiosynthese (DE-588)4175987-4 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4342432-6 (DE-588)4175987-4 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Cell-Free Protein Synthesis Methods and Protocols |
| title_auth | Cell-Free Protein Synthesis Methods and Protocols |
| title_exact_search | Cell-Free Protein Synthesis Methods and Protocols |
| title_full | Cell-Free Protein Synthesis Methods and Protocols edited by Kirill Alexandrov, Wayne A. Johnston |
| title_fullStr | Cell-Free Protein Synthesis Methods and Protocols edited by Kirill Alexandrov, Wayne A. Johnston |
| title_full_unstemmed | Cell-Free Protein Synthesis Methods and Protocols edited by Kirill Alexandrov, Wayne A. Johnston |
| title_short | Cell-Free Protein Synthesis |
| title_sort | cell free protein synthesis methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Protein Science Life sciences Proteins Zellfreies System (DE-588)4342432-6 gnd Proteinbiosynthese (DE-588)4175987-4 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Life Sciences Protein Science Life sciences Proteins Zellfreies System Proteinbiosynthese Methode Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-62703-782-2 |
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