Membrane Protein Structure Determination: Methods and Protocols
Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membran...
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Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2010
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Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
654 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology |
Beschreibung: | 1 Online-Ressource (XII, 459 p. 101 illus., 14 illus. in color) |
ISBN: | 9781607617624 |
DOI: | 10.1007/978-1-60761-762-4 |
Internformat
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520 | |a Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology | ||
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Datensatz im Suchindex
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any_adam_object | |
author2 | Lacapère, Jean-Jacques |
author2_role | edt |
author2_variant | j j l jjl |
author_facet | Lacapère, Jean-Jacques |
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dewey-full | 572.6 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 572 - Biochemistry |
dewey-raw | 572.6 |
dewey-search | 572.6 |
dewey-sort | 3572.6 |
dewey-tens | 570 - Biology |
discipline | Biologie |
doi_str_mv | 10.1007/978-1-60761-762-4 |
format | Electronic eBook |
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language | English |
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spelling | Membrane Protein Structure Determination Methods and Protocols edited by Jean-Jacques Lacapère Totowa, NJ Humana Press 2010 1 Online-Ressource (XII, 459 p. 101 illus., 14 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 654 Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology Life Sciences Protein Science Membrane Biology Life sciences Proteins Cell membranes Strukturanalyse (DE-588)4183787-3 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf Membranproteine (DE-588)4130026-9 s Strukturanalyse (DE-588)4183787-3 s Methode (DE-588)4038971-6 s b DE-604 Lacapère, Jean-Jacques edt Erscheint auch als Druck-Ausgabe 9781607617617 https://doi.org/10.1007/978-1-60761-762-4 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Membrane Protein Structure Determination Methods and Protocols Life Sciences Protein Science Membrane Biology Life sciences Proteins Cell membranes Strukturanalyse (DE-588)4183787-3 gnd Methode (DE-588)4038971-6 gnd Membranproteine (DE-588)4130026-9 gnd |
subject_GND | (DE-588)4183787-3 (DE-588)4038971-6 (DE-588)4130026-9 |
title | Membrane Protein Structure Determination Methods and Protocols |
title_auth | Membrane Protein Structure Determination Methods and Protocols |
title_exact_search | Membrane Protein Structure Determination Methods and Protocols |
title_full | Membrane Protein Structure Determination Methods and Protocols edited by Jean-Jacques Lacapère |
title_fullStr | Membrane Protein Structure Determination Methods and Protocols edited by Jean-Jacques Lacapère |
title_full_unstemmed | Membrane Protein Structure Determination Methods and Protocols edited by Jean-Jacques Lacapère |
title_short | Membrane Protein Structure Determination |
title_sort | membrane protein structure determination methods and protocols |
title_sub | Methods and Protocols |
topic | Life Sciences Protein Science Membrane Biology Life sciences Proteins Cell membranes Strukturanalyse (DE-588)4183787-3 gnd Methode (DE-588)4038971-6 gnd Membranproteine (DE-588)4130026-9 gnd |
topic_facet | Life Sciences Protein Science Membrane Biology Life sciences Proteins Cell membranes Strukturanalyse Methode Membranproteine |
url | https://doi.org/10.1007/978-1-60761-762-4 |
work_keys_str_mv | AT lacaperejeanjacques membraneproteinstructuredeterminationmethodsandprotocols |