Gel-Free Proteomics: Methods and Protocols

Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five c...

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Weitere Verfasser: Gevaert, Kris (HerausgeberIn), Vandekerckhove, Joël (HerausgeberIn)
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: Totowa, NJ Humana Press 2011
Schriftenreihe:Methods in Molecular Biology, Methods and Protocols 753
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Zusammenfassung:Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Gel-Free Proteomics: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this critical field
Beschreibung:1 Online-Ressource (XII, 400 p. 84 illus)
ISBN:9781617791482
DOI:10.1007/978-1-61779-148-2

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