Antiviral Resistance in Plants: Methods and Protocols
Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2012
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
894 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait |
| Beschreibung: | 1 Online-Ressource (XI, 380 p. 59 illus., 10 illus. in color) |
| ISBN: | 9781617798825 |
| DOI: | 10.1007/978-1-61779-882-5 |
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| 520 | |a Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Plant Sciences | |
| 650 | 4 | |a Plant Pathology | |
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Datensatz im Suchindex
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| dewey-full | 580 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 580 - Plants |
| dewey-raw | 580 |
| dewey-search | 580 |
| dewey-sort | 3580 |
| dewey-tens | 580 - Plants |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-61779-882-5 |
| format | Electronic eBook |
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| id | DE-604.BV044951972 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:36Z |
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| isbn | 9781617798825 |
| language | English |
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| spelling | Antiviral Resistance in Plants Methods and Protocols edited by John M. Watson, Ming-Bo Wang Totowa, NJ Humana Press 2012 1 Online-Ressource (XI, 380 p. 59 illus., 10 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 894 Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait Life Sciences Plant Sciences Plant Pathology Virology Life sciences Plant science Botany Plant pathology Watson, John M. edt Wang, Ming-Bo edt Erscheint auch als Druck-Ausgabe 9781617798818 https://doi.org/10.1007/978-1-61779-882-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Antiviral Resistance in Plants Methods and Protocols Life Sciences Plant Sciences Plant Pathology Virology Life sciences Plant science Botany Plant pathology |
| title | Antiviral Resistance in Plants Methods and Protocols |
| title_auth | Antiviral Resistance in Plants Methods and Protocols |
| title_exact_search | Antiviral Resistance in Plants Methods and Protocols |
| title_full | Antiviral Resistance in Plants Methods and Protocols edited by John M. Watson, Ming-Bo Wang |
| title_fullStr | Antiviral Resistance in Plants Methods and Protocols edited by John M. Watson, Ming-Bo Wang |
| title_full_unstemmed | Antiviral Resistance in Plants Methods and Protocols edited by John M. Watson, Ming-Bo Wang |
| title_short | Antiviral Resistance in Plants |
| title_sort | antiviral resistance in plants methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Plant Sciences Plant Pathology Virology Life sciences Plant science Botany Plant pathology |
| topic_facet | Life Sciences Plant Sciences Plant Pathology Virology Life sciences Plant science Botany Plant pathology |
| url | https://doi.org/10.1007/978-1-61779-882-5 |
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