Inositol Phosphates and Lipids: Methods and Protocols
Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, ex...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2010
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
645 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide |
| Beschreibung: | 1 Online-Ressource (X, 230 p. 54 illus) |
| ISBN: | 9781603271752 |
| DOI: | 10.1007/978-1-60327-175-2 |
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| 520 | |a Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide | ||
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| indexdate | 2024-07-10T08:05:36Z |
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| isbn | 9781603271752 |
| language | English |
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| spelling | Inositol Phosphates and Lipids Methods and Protocols edited by Christopher J. Barker Totowa, NJ Humana Press 2010 1 Online-Ressource (X, 230 p. 54 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 645 Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide Life Sciences Cell Biology Cell Physiology Life sciences Cell biology Cell physiology Barker, Christopher J. edt Erscheint auch als Druck-Ausgabe 9781603271745 https://doi.org/10.1007/978-1-60327-175-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Inositol Phosphates and Lipids Methods and Protocols Life Sciences Cell Biology Cell Physiology Life sciences Cell biology Cell physiology |
| title | Inositol Phosphates and Lipids Methods and Protocols |
| title_auth | Inositol Phosphates and Lipids Methods and Protocols |
| title_exact_search | Inositol Phosphates and Lipids Methods and Protocols |
| title_full | Inositol Phosphates and Lipids Methods and Protocols edited by Christopher J. Barker |
| title_fullStr | Inositol Phosphates and Lipids Methods and Protocols edited by Christopher J. Barker |
| title_full_unstemmed | Inositol Phosphates and Lipids Methods and Protocols edited by Christopher J. Barker |
| title_short | Inositol Phosphates and Lipids |
| title_sort | inositol phosphates and lipids methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Cell Physiology Life sciences Cell biology Cell physiology |
| topic_facet | Life Sciences Cell Biology Cell Physiology Life sciences Cell biology Cell physiology |
| url | https://doi.org/10.1007/978-1-60327-175-2 |
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