Handbook of ELISPOT: Methods and Protocols
Although the ELISPOT (enzyme-linked immunospot) assay is widely used in many fields of biomedical research, producing recognizable and quantifiable spots may be very difficult not only for beginners, but also for experienced researchers. In Handbook of ELISPOT: Methods and Protocols, the first book...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2005
|
| Schriftenreihe: | Methods in Molecular Biology™
302 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Although the ELISPOT (enzyme-linked immunospot) assay is widely used in many fields of biomedical research, producing recognizable and quantifiable spots may be very difficult not only for beginners, but also for experienced researchers. In Handbook of ELISPOT: Methods and Protocols, the first book dedicated entirely to the ELISPOT, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Handbook of ELISPOT: Methods and Protocols offers both novice and experienced researchers a wealth of easy-to-understand information that will clarify the technically challenging aspects of ELISPOT and open the door to its wider successful use |
| Beschreibung: | 1 Online-Ressource (XIV, 322 p. 123 illus., 10 illus. in color) |
| ISBN: | 9781592599035 |
| DOI: | 10.1385/1592599036 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044951924 | ||
| 003 | DE-604 | ||
| 005 | 20180928 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2005 |||| o||u| ||||||eng d | ||
| 020 | |a 9781592599035 |9 978-1-59259-903-5 | ||
| 024 | 7 | |a 10.1385/1592599036 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59259-903-5 | ||
| 035 | |a (OCoLC)712421441 | ||
| 035 | |a (DE-599)BVBBV044951924 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 050 | 0 | |a QP519.9.E48 | |
| 082 | 0 | |a 616.07/9 |2 22 | |
| 082 | 0 | |a 616.079 |2 23 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a WC 4350 |0 (DE-625)148095: |2 rvk | ||
| 084 | |a YV 4500 |0 (DE-625)154224:12905 |2 rvk | ||
| 245 | 1 | 0 | |a Handbook of ELISPOT |b Methods and Protocols |c edited by Alexander E. Kalyuzhny |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2005 | |
| 300 | |a 1 Online-Ressource (XIV, 322 p. 123 illus., 10 illus. in color) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology™ |v 302 | |
| 520 | |a Although the ELISPOT (enzyme-linked immunospot) assay is widely used in many fields of biomedical research, producing recognizable and quantifiable spots may be very difficult not only for beginners, but also for experienced researchers. In Handbook of ELISPOT: Methods and Protocols, the first book dedicated entirely to the ELISPOT, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Handbook of ELISPOT: Methods and Protocols offers both novice and experienced researchers a wealth of easy-to-understand information that will clarify the technically challenging aspects of ELISPOT and open the door to its wider successful use | ||
| 650 | 4 | |a Biomedicine | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Medicine | |
| 650 | 4 | |a Immunology | |
| 650 | 0 | 7 | |a Enzyme-linked immunosorbent assay |0 (DE-588)4152476-7 |2 gnd |9 rswk-swf |
| 655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Enzyme-linked immunosorbent assay |0 (DE-588)4152476-7 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Kalyuzhny, Alexander E. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781588294692 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/1592599036 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030344680 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1385/1592599036 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1385/1592599036 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178541168820224 |
|---|---|
| any_adam_object | |
| author2 | Kalyuzhny, Alexander E. |
| author2_role | edt |
| author2_variant | a e k ae aek |
| author_facet | Kalyuzhny, Alexander E. |
| building | Verbundindex |
| bvnumber | BV044951924 |
| callnumber-first | Q - Science |
| callnumber-label | QP519 |
| callnumber-raw | QP519.9.E48 |
| callnumber-search | QP519.9.E48 |
| callnumber-sort | QP 3519.9 E48 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 4150 WC 4350 YV 4500 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59259-903-5 (OCoLC)712421441 (DE-599)BVBBV044951924 |
| dewey-full | 616.07/9 616.079 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.07/9 616.079 |
| dewey-search | 616.07/9 616.079 |
| dewey-sort | 3616.07 19 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| doi_str_mv | 10.1385/1592599036 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03678nmm a2200541zcb4500</leader><controlfield tag="001">BV044951924</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180928 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2005 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781592599035</subfield><subfield code="9">978-1-59259-903-5</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/1592599036</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59259-903-5</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)712421441</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044951924</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QP519.9.E48</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">616.07/9</subfield><subfield code="2">22</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">616.079</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4350</subfield><subfield code="0">(DE-625)148095:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">YV 4500</subfield><subfield code="0">(DE-625)154224:12905</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Handbook of ELISPOT</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Alexander E. Kalyuzhny</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2005</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XIV, 322 p. 123 illus., 10 illus. in color)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology™</subfield><subfield code="v">302</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Although the ELISPOT (enzyme-linked immunospot) assay is widely used in many fields of biomedical research, producing recognizable and quantifiable spots may be very difficult not only for beginners, but also for experienced researchers. In Handbook of ELISPOT: Methods and Protocols, the first book dedicated entirely to the ELISPOT, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Handbook of ELISPOT: Methods and Protocols offers both novice and experienced researchers a wealth of easy-to-understand information that will clarify the technically challenging aspects of ELISPOT and open the door to its wider successful use</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biomedicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Medicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunology</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Enzyme-linked immunosorbent assay</subfield><subfield code="0">(DE-588)4152476-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Enzyme-linked immunosorbent assay</subfield><subfield code="0">(DE-588)4152476-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kalyuzhny, Alexander E.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781588294692</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/1592599036</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030344680</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/1592599036</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/1592599036</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV044951924 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:36Z |
| institution | BVB |
| isbn | 9781592599035 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030344680 |
| oclc_num | 712421441 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XIV, 322 p. 123 illus., 10 illus. in color) |
| psigel | ZDB-2-PRO |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology™ |
| spelling | Handbook of ELISPOT Methods and Protocols edited by Alexander E. Kalyuzhny Totowa, NJ Humana Press 2005 1 Online-Ressource (XIV, 322 p. 123 illus., 10 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 302 Although the ELISPOT (enzyme-linked immunospot) assay is widely used in many fields of biomedical research, producing recognizable and quantifiable spots may be very difficult not only for beginners, but also for experienced researchers. In Handbook of ELISPOT: Methods and Protocols, the first book dedicated entirely to the ELISPOT, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Handbook of ELISPOT: Methods and Protocols offers both novice and experienced researchers a wealth of easy-to-understand information that will clarify the technically challenging aspects of ELISPOT and open the door to its wider successful use Biomedicine Immunology Medicine Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Enzyme-linked immunosorbent assay (DE-588)4152476-7 s DE-604 Kalyuzhny, Alexander E. edt Erscheint auch als Druck-Ausgabe 9781588294692 https://doi.org/10.1385/1592599036 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Handbook of ELISPOT Methods and Protocols Biomedicine Immunology Medicine Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd |
| subject_GND | (DE-588)4152476-7 (DE-588)4143413-4 |
| title | Handbook of ELISPOT Methods and Protocols |
| title_auth | Handbook of ELISPOT Methods and Protocols |
| title_exact_search | Handbook of ELISPOT Methods and Protocols |
| title_full | Handbook of ELISPOT Methods and Protocols edited by Alexander E. Kalyuzhny |
| title_fullStr | Handbook of ELISPOT Methods and Protocols edited by Alexander E. Kalyuzhny |
| title_full_unstemmed | Handbook of ELISPOT Methods and Protocols edited by Alexander E. Kalyuzhny |
| title_short | Handbook of ELISPOT |
| title_sort | handbook of elispot methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Immunology Medicine Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd |
| topic_facet | Biomedicine Immunology Medicine Enzyme-linked immunosorbent assay Aufsatzsammlung |
| url | https://doi.org/10.1385/1592599036 |
| work_keys_str_mv | AT kalyuzhnyalexandere handbookofelispotmethodsandprotocols |