Capillary Electrophoresis of Proteins and Peptides:
Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leadin...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Schriftenreihe: | Methods in Molecular Biology™
276 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development |
| Beschreibung: | 1 Online-Ressource (XI, 332 p) |
| ISBN: | 9781592597987 |
| DOI: | 10.1385/159259798X |
Internformat
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| 520 | |a Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development | ||
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Datensatz im Suchindex
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| spelling | Capillary Electrophoresis of Proteins and Peptides edited by Mark A. Strege, Avinash L. Lagu Totowa, NJ Humana Press 2004 1 Online-Ressource (XI, 332 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 276 Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development Life Sciences Biochemistry, general Life sciences Biochemistry Labortechnik (DE-588)4123602-6 gnd rswk-swf Peptide (DE-588)4045125-2 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Kapillarelektrophorese (DE-588)4290002-5 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Kapillarelektrophorese (DE-588)4290002-5 s Labortechnik (DE-588)4123602-6 s DE-604 Peptide (DE-588)4045125-2 s b DE-604 Strege, Mark A. edt Lagu, Avinash L. edt Erscheint auch als Druck-Ausgabe 9781588290175 https://doi.org/10.1385/159259798X Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Capillary Electrophoresis of Proteins and Peptides Life Sciences Biochemistry, general Life sciences Biochemistry Labortechnik (DE-588)4123602-6 gnd Peptide (DE-588)4045125-2 gnd Proteine (DE-588)4076388-2 gnd Kapillarelektrophorese (DE-588)4290002-5 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4045125-2 (DE-588)4076388-2 (DE-588)4290002-5 (DE-588)4143413-4 |
| title | Capillary Electrophoresis of Proteins and Peptides |
| title_auth | Capillary Electrophoresis of Proteins and Peptides |
| title_exact_search | Capillary Electrophoresis of Proteins and Peptides |
| title_full | Capillary Electrophoresis of Proteins and Peptides edited by Mark A. Strege, Avinash L. Lagu |
| title_fullStr | Capillary Electrophoresis of Proteins and Peptides edited by Mark A. Strege, Avinash L. Lagu |
| title_full_unstemmed | Capillary Electrophoresis of Proteins and Peptides edited by Mark A. Strege, Avinash L. Lagu |
| title_short | Capillary Electrophoresis of Proteins and Peptides |
| title_sort | capillary electrophoresis of proteins and peptides |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Labortechnik (DE-588)4123602-6 gnd Peptide (DE-588)4045125-2 gnd Proteine (DE-588)4076388-2 gnd Kapillarelektrophorese (DE-588)4290002-5 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Labortechnik Peptide Proteine Kapillarelektrophorese Aufsatzsammlung |
| url | https://doi.org/10.1385/159259798X |
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