Drosophila Cytogenetics Protocols:
Probably no single experimental organism has contributed as much to our understanding of the structure, function, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-st...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Schriftenreihe: | Methods in Molecular Biology
247 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Probably no single experimental organism has contributed as much to our understanding of the structure, function, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-step detail, all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. General areas covered include analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated cell sorting of ovarian follicle cells, analyses of nuclear division cycles in embryos, and analyses of programmed cell death. In addition, there are conventional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to both polytene and mitotic chromosomes. A cutting-edge combined FISH and immunolocalization protocol will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression. Each readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Readily accessible and highly practical, Drosophila Cytogenetics Protocols provides a comprehensive cytogenetics laboratory manual for Drosophila investigators, one that is suitable not only for novices, but also highly informative for seasoned investigators |
| Beschreibung: | 1 Online-Ressource (XIII, 468 p. 269 illus., 30 illus. in color) |
| ISBN: | 9781592596652 |
| DOI: | 10.1385/1592596657 |
Internformat
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| 520 | |a Probably no single experimental organism has contributed as much to our understanding of the structure, function, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-step detail, all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. General areas covered include analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated cell sorting of ovarian follicle cells, analyses of nuclear division cycles in embryos, and analyses of programmed cell death. In addition, there are conventional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to both polytene and mitotic chromosomes. A cutting-edge combined FISH and immunolocalization protocol will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression. Each readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Readily accessible and highly practical, Drosophila Cytogenetics Protocols provides a comprehensive cytogenetics laboratory manual for Drosophila investigators, one that is suitable not only for novices, but also highly informative for seasoned investigators | ||
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Datensatz im Suchindex
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| dewey-tens | 590 - Animals |
| discipline | Biologie |
| doi_str_mv | 10.1385/1592596657 |
| format | Electronic eBook |
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| spelling | Drosophila Cytogenetics Protocols edited by Daryl S. Henderson Totowa, NJ Humana Press 2004 1 Online-Ressource (XIII, 468 p. 269 illus., 30 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 247 Probably no single experimental organism has contributed as much to our understanding of the structure, function, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-step detail, all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. General areas covered include analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated cell sorting of ovarian follicle cells, analyses of nuclear division cycles in embryos, and analyses of programmed cell death. In addition, there are conventional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to both polytene and mitotic chromosomes. A cutting-edge combined FISH and immunolocalization protocol will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression. Each readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Readily accessible and highly practical, Drosophila Cytogenetics Protocols provides a comprehensive cytogenetics laboratory manual for Drosophila investigators, one that is suitable not only for novices, but also highly informative for seasoned investigators Life Sciences Animal Genetics and Genomics Life sciences Animal genetics Taufliege (DE-588)4078168-9 gnd rswk-swf Cytogenetik (DE-588)4070176-1 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Taufliege (DE-588)4078168-9 s Cytogenetik (DE-588)4070176-1 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Henderson, Daryl S. edt Erscheint auch als Druck-Ausgabe 9781588290502 https://doi.org/10.1385/1592596657 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Drosophila Cytogenetics Protocols Life Sciences Animal Genetics and Genomics Life sciences Animal genetics Taufliege (DE-588)4078168-9 gnd Cytogenetik (DE-588)4070176-1 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| subject_GND | (DE-588)4078168-9 (DE-588)4070176-1 (DE-588)4324907-3 (DE-588)4143413-4 |
| title | Drosophila Cytogenetics Protocols |
| title_auth | Drosophila Cytogenetics Protocols |
| title_exact_search | Drosophila Cytogenetics Protocols |
| title_full | Drosophila Cytogenetics Protocols edited by Daryl S. Henderson |
| title_fullStr | Drosophila Cytogenetics Protocols edited by Daryl S. Henderson |
| title_full_unstemmed | Drosophila Cytogenetics Protocols edited by Daryl S. Henderson |
| title_short | Drosophila Cytogenetics Protocols |
| title_sort | drosophila cytogenetics protocols |
| topic | Life Sciences Animal Genetics and Genomics Life sciences Animal genetics Taufliege (DE-588)4078168-9 gnd Cytogenetik (DE-588)4070176-1 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| topic_facet | Life Sciences Animal Genetics and Genomics Life sciences Animal genetics Taufliege Cytogenetik Untersuchungsmethode Aufsatzsammlung |
| url | https://doi.org/10.1385/1592596657 |
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