Skeletal Muscle Regeneration in the Mouse: Methods and Protocols
This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a researc...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2016
|
| Schriftenreihe: | Methods in Molecular Biology
1460 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system |
| Beschreibung: | 1 Online-Ressource (XII, 346 p. 97 illus., 87 illus. in color) |
| ISBN: | 9781493938100 |
| DOI: | 10.1007/978-1-4939-3810-0 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044951879 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2016 |||| o||u| ||||||eng d | ||
| 020 | |a 9781493938100 |9 978-1-4939-3810-0 | ||
| 024 | 7 | |a 10.1007/978-1-4939-3810-0 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-4939-3810-0 | ||
| 035 | |a (OCoLC)960419013 | ||
| 035 | |a (DE-599)BVBBV044951879 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 611.01816 |2 23 | |
| 245 | 1 | 0 | |a Skeletal Muscle Regeneration in the Mouse |b Methods and Protocols |c edited by Michael Kyba |
| 264 | 1 | |a New York, NY |b Springer New York |c 2016 | |
| 300 | |a 1 Online-Ressource (XII, 346 p. 97 illus., 87 illus. in color) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology |v 1460 | |
| 520 | |a This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system | ||
| 650 | 4 | |a Biomedicine | |
| 650 | 4 | |a Molecular Medicine | |
| 650 | 4 | |a Medicine | |
| 650 | 4 | |a Molecular biology | |
| 700 | 1 | |a Kyba, Michael |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781493938087 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-3810-0 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030344635 | ||
| 966 | e | |u https://doi.org/10.1007/978-1-4939-3810-0 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-4939-3810-0 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178541062914048 |
|---|---|
| any_adam_object | |
| author2 | Kyba, Michael |
| author2_role | edt |
| author2_variant | m k mk |
| author_facet | Kyba, Michael |
| building | Verbundindex |
| bvnumber | BV044951879 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-4939-3810-0 (OCoLC)960419013 (DE-599)BVBBV044951879 |
| dewey-full | 611.01816 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 611 - Human anatomy, cytology, histology |
| dewey-raw | 611.01816 |
| dewey-search | 611.01816 |
| dewey-sort | 3611.01816 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| doi_str_mv | 10.1007/978-1-4939-3810-0 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03081nmm a2200421zcb4500</leader><controlfield tag="001">BV044951879</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2016 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781493938100</subfield><subfield code="9">978-1-4939-3810-0</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-4939-3810-0</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-4939-3810-0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)960419013</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044951879</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">611.01816</subfield><subfield code="2">23</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Skeletal Muscle Regeneration in the Mouse</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Michael Kyba</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Springer New York</subfield><subfield code="c">2016</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XII, 346 p. 97 illus., 87 illus. in color)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield><subfield code="v">1460</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biomedicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Molecular Medicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Medicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Molecular biology</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kyba, Michael</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781493938087</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-4939-3810-0</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030344635</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-3810-0</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-3810-0</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV044951879 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:36Z |
| institution | BVB |
| isbn | 9781493938100 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030344635 |
| oclc_num | 960419013 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XII, 346 p. 97 illus., 87 illus. in color) |
| psigel | ZDB-2-PRO |
| publishDate | 2016 |
| publishDateSearch | 2016 |
| publishDateSort | 2016 |
| publisher | Springer New York |
| record_format | marc |
| series2 | Methods in Molecular Biology |
| spelling | Skeletal Muscle Regeneration in the Mouse Methods and Protocols edited by Michael Kyba New York, NY Springer New York 2016 1 Online-Ressource (XII, 346 p. 97 illus., 87 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1460 This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system Biomedicine Molecular Medicine Medicine Molecular biology Kyba, Michael edt Erscheint auch als Druck-Ausgabe 9781493938087 https://doi.org/10.1007/978-1-4939-3810-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Skeletal Muscle Regeneration in the Mouse Methods and Protocols Biomedicine Molecular Medicine Medicine Molecular biology |
| title | Skeletal Muscle Regeneration in the Mouse Methods and Protocols |
| title_auth | Skeletal Muscle Regeneration in the Mouse Methods and Protocols |
| title_exact_search | Skeletal Muscle Regeneration in the Mouse Methods and Protocols |
| title_full | Skeletal Muscle Regeneration in the Mouse Methods and Protocols edited by Michael Kyba |
| title_fullStr | Skeletal Muscle Regeneration in the Mouse Methods and Protocols edited by Michael Kyba |
| title_full_unstemmed | Skeletal Muscle Regeneration in the Mouse Methods and Protocols edited by Michael Kyba |
| title_short | Skeletal Muscle Regeneration in the Mouse |
| title_sort | skeletal muscle regeneration in the mouse methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Molecular Medicine Medicine Molecular biology |
| topic_facet | Biomedicine Molecular Medicine Medicine Molecular biology |
| url | https://doi.org/10.1007/978-1-4939-3810-0 |
| work_keys_str_mv | AT kybamichael skeletalmuscleregenerationinthemousemethodsandprotocols |