Enzymatic Analysis: A Practical Guide
Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refin...
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| Hauptverfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1993
|
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step |
| Beschreibung: | 1 Online-Ressource (X, 403 p) |
| ISBN: | 9781603274074 |
| DOI: | 10.1007/978-1-60327-407-4 |
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| dewey-full | 572 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
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| dewey-search | 572 |
| dewey-sort | 3572 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-60327-407-4 |
| format | Electronic eBook |
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| language | English |
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| spelling | Passonneau, Janet V. Verfasser aut Enzymatic Analysis A Practical Guide by Janet V. Passonneau, Oliver H. Lowry Totowa, NJ Humana Press 1993 1 Online-Ressource (X, 403 p) txt rdacontent c rdamedia cr rdacarrier Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step Life Sciences Biochemistry, general Life sciences Biochemistry Enzymatische Analyse (DE-588)4014989-4 gnd rswk-swf Enzymatische Analyse (DE-588)4014989-4 s 1\p DE-604 Lowry, Oliver H. aut Erscheint auch als Druck-Ausgabe 9780896032385 https://doi.org/10.1007/978-1-60327-407-4 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Passonneau, Janet V. Lowry, Oliver H. Enzymatic Analysis A Practical Guide Life Sciences Biochemistry, general Life sciences Biochemistry Enzymatische Analyse (DE-588)4014989-4 gnd |
| subject_GND | (DE-588)4014989-4 |
| title | Enzymatic Analysis A Practical Guide |
| title_auth | Enzymatic Analysis A Practical Guide |
| title_exact_search | Enzymatic Analysis A Practical Guide |
| title_full | Enzymatic Analysis A Practical Guide by Janet V. Passonneau, Oliver H. Lowry |
| title_fullStr | Enzymatic Analysis A Practical Guide by Janet V. Passonneau, Oliver H. Lowry |
| title_full_unstemmed | Enzymatic Analysis A Practical Guide by Janet V. Passonneau, Oliver H. Lowry |
| title_short | Enzymatic Analysis |
| title_sort | enzymatic analysis a practical guide |
| title_sub | A Practical Guide |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Enzymatische Analyse (DE-588)4014989-4 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Enzymatische Analyse |
| url | https://doi.org/10.1007/978-1-60327-407-4 |
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