Thermogenic Fat: Methods and Protocols
This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, a...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
|
| Schriftenreihe: | Methods in Molecular Biology
1566 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions |
| Beschreibung: | 1 Online-Ressource (XI, 214 p. 42 illus., 30 illus. in color) |
| ISBN: | 9781493968206 |
| DOI: | 10.1007/978-1-4939-6820-6 |
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| 520 | |a This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions | ||
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Datensatz im Suchindex
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| indexdate | 2024-07-10T08:05:36Z |
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| isbn | 9781493968206 |
| language | English |
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| physical | 1 Online-Ressource (XI, 214 p. 42 illus., 30 illus. in color) |
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| spelling | Thermogenic Fat Methods and Protocols edited by Jun Wu New York, NY Springer New York 2017 1 Online-Ressource (XI, 214 p. 42 illus., 30 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1566 This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions Life Sciences Cell Biology Life sciences Cell biology Wu, Jun edt Erscheint auch als Druck-Ausgabe 9781493968190 https://doi.org/10.1007/978-1-4939-6820-6 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Thermogenic Fat Methods and Protocols Life Sciences Cell Biology Life sciences Cell biology |
| title | Thermogenic Fat Methods and Protocols |
| title_auth | Thermogenic Fat Methods and Protocols |
| title_exact_search | Thermogenic Fat Methods and Protocols |
| title_full | Thermogenic Fat Methods and Protocols edited by Jun Wu |
| title_fullStr | Thermogenic Fat Methods and Protocols edited by Jun Wu |
| title_full_unstemmed | Thermogenic Fat Methods and Protocols edited by Jun Wu |
| title_short | Thermogenic Fat |
| title_sort | thermogenic fat methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology |
| url | https://doi.org/10.1007/978-1-4939-6820-6 |
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