E. coli Gene Expression Protocols:
Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to qu...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2003
|
| Schriftenreihe: | Methods in Molecular Biology™
205 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics |
| Beschreibung: | 1 Online-Ressource (XI, 347 p. 129 illus) |
| ISBN: | 9781592593019 |
| DOI: | 10.1385/1592593011 |
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| 520 | |a Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics | ||
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| spelling | E. coli Gene Expression Protocols edited by Peter E. Vaillancourt Totowa, NJ Humana Press 2003 1 Online-Ressource (XI, 347 p. 129 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 205 Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Genexpression (DE-588)4020136-3 gnd rswk-swf Escherichia coli (DE-588)4070959-0 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Escherichia coli (DE-588)4070959-0 s Genexpression (DE-588)4020136-3 s DE-604 Vaillancourt, Peter E. edt Erscheint auch als Druck-Ausgabe 9781588290083 https://doi.org/10.1385/1592593011 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | E. coli Gene Expression Protocols Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Genexpression (DE-588)4020136-3 gnd Escherichia coli (DE-588)4070959-0 gnd |
| subject_GND | (DE-588)4020136-3 (DE-588)4070959-0 (DE-588)4143413-4 |
| title | E. coli Gene Expression Protocols |
| title_auth | E. coli Gene Expression Protocols |
| title_exact_search | E. coli Gene Expression Protocols |
| title_full | E. coli Gene Expression Protocols edited by Peter E. Vaillancourt |
| title_fullStr | E. coli Gene Expression Protocols edited by Peter E. Vaillancourt |
| title_full_unstemmed | E. coli Gene Expression Protocols edited by Peter E. Vaillancourt |
| title_short | E. coli Gene Expression Protocols |
| title_sort | e coli gene expression protocols |
| topic | Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Genexpression (DE-588)4020136-3 gnd Escherichia coli (DE-588)4070959-0 gnd |
| topic_facet | Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Genexpression Escherichia coli Aufsatzsammlung |
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