Microbial Processes and Products:
The development of recombinant DNA techniques over the last 20 years has greatly expanded the opportunities for using microorganisms to produce a broad range of valuable substances. In Microbial Processes and Products, outstanding leaders in using microorganisms as cell factories describe in detail...
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Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2005
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Schriftenreihe: | Methods in Biotechnology
18 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
Zusammenfassung: | The development of recombinant DNA techniques over the last 20 years has greatly expanded the opportunities for using microorganisms to produce a broad range of valuable substances. In Microbial Processes and Products, outstanding leaders in using microorganisms as cell factories describe in detail their best laboratory procedures for many processes and products mediated by microorganisms. An overview chapter describes how to develop strain improvement programs and strategies to optimize fermentation processes. Taking advantage of the most recent developments in such processes, the authors offer step-by-step experimental methods for the optimal design of microbial metabolite production, including semisynthetic derivatives of cephalosporins, erythromycin, antitumor compounds, plasmids for gene therapy and DNA vaccination, L-lysine, vitamins B2 and B12, the sweet-tasting protein thaumatin, the carotenoids b-carotene and astaxanthin, the polysaccharide gellan, and bacteria-producing bacteria for sausage fermentation. Additionally, the use of phenylacetyl-CoA catabolon for enzymatic synthesis of penicillins, aromatic biotransformations, synthesis of new bioplastics, biosensor design, or synthesis of drug vehicles, and the development of a phosphate encoding gene as a reporter and to monitor gene expression are illustrated. The diverse chemicals and biochemicals produced can be used in human health, nutrition, and environmental protection. Additional chapters offer techniques for analysis of antimicrobial metabolites and carotenoids, volatile sulfur compounds, metabolic pathway fluxes, gene expression arrays, proteome analysis, bacterial modulation of the innate immune response, bioleaching activity, and heavy metal remediation. Finally, three overview chapters on transport of biological material, deposit of biological material for patent purposes, and protection of biotechnological inventions are shown. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Enzymes and Biotransformations, describes in detail cutting-edge techniques for the screening, evolution, production, immobilization, and use of enzymes. |
Beschreibung: | 1 Online-Ressource (XIV, 516 p) |
ISBN: | 9781592598472 |
DOI: | 10.1385/1592598471 |
Internformat
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520 | |a Taking advantage of the most recent developments in such processes, the authors offer step-by-step experimental methods for the optimal design of microbial metabolite production, including semisynthetic derivatives of cephalosporins, erythromycin, antitumor compounds, plasmids for gene therapy and DNA vaccination, L-lysine, vitamins B2 and B12, the sweet-tasting protein thaumatin, the carotenoids b-carotene and astaxanthin, the polysaccharide gellan, and bacteria-producing bacteria for sausage fermentation. Additionally, the use of phenylacetyl-CoA catabolon for enzymatic synthesis of penicillins, aromatic biotransformations, synthesis of new bioplastics, biosensor design, or synthesis of drug vehicles, and the development of a phosphate encoding gene as a reporter and to monitor gene expression are illustrated. The diverse chemicals and biochemicals produced can be used in human health, nutrition, and environmental protection. | ||
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Datensatz im Suchindex
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---|---|
any_adam_object | |
author2 | Barredo, José-Luis |
author2_role | edt |
author2_variant | j l b jlb |
author_facet | Barredo, José-Luis |
building | Verbundindex |
bvnumber | BV044951832 |
classification_rvk | WF 9700 |
collection | ZDB-2-PRO |
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dewey-full | 579 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 579 - Microorganisms, fungi & algae |
dewey-raw | 579 |
dewey-search | 579 |
dewey-sort | 3579 |
dewey-tens | 570 - Biology |
discipline | Biologie |
doi_str_mv | 10.1385/1592598471 |
format | Electronic eBook |
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isbn | 9781592598472 |
language | English |
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spelling | Microbial Processes and Products edited by José-Luis Barredo Totowa, NJ Humana Press 2005 1 Online-Ressource (XIV, 516 p) txt rdacontent c rdamedia cr rdacarrier Methods in Biotechnology 18 The development of recombinant DNA techniques over the last 20 years has greatly expanded the opportunities for using microorganisms to produce a broad range of valuable substances. In Microbial Processes and Products, outstanding leaders in using microorganisms as cell factories describe in detail their best laboratory procedures for many processes and products mediated by microorganisms. An overview chapter describes how to develop strain improvement programs and strategies to optimize fermentation processes. Taking advantage of the most recent developments in such processes, the authors offer step-by-step experimental methods for the optimal design of microbial metabolite production, including semisynthetic derivatives of cephalosporins, erythromycin, antitumor compounds, plasmids for gene therapy and DNA vaccination, L-lysine, vitamins B2 and B12, the sweet-tasting protein thaumatin, the carotenoids b-carotene and astaxanthin, the polysaccharide gellan, and bacteria-producing bacteria for sausage fermentation. Additionally, the use of phenylacetyl-CoA catabolon for enzymatic synthesis of penicillins, aromatic biotransformations, synthesis of new bioplastics, biosensor design, or synthesis of drug vehicles, and the development of a phosphate encoding gene as a reporter and to monitor gene expression are illustrated. The diverse chemicals and biochemicals produced can be used in human health, nutrition, and environmental protection. Additional chapters offer techniques for analysis of antimicrobial metabolites and carotenoids, volatile sulfur compounds, metabolic pathway fluxes, gene expression arrays, proteome analysis, bacterial modulation of the innate immune response, bioleaching activity, and heavy metal remediation. Finally, three overview chapters on transport of biological material, deposit of biological material for patent purposes, and protection of biotechnological inventions are shown. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Enzymes and Biotransformations, describes in detail cutting-edge techniques for the screening, evolution, production, immobilization, and use of enzymes. Life Sciences Microbiology Life sciences Mikroorganismus (DE-588)4039226-0 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Industrielle Mikrobiologie (DE-588)4026845-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Biotechnologie (DE-588)4069491-4 s Mikroorganismus (DE-588)4039226-0 s 2\p DE-604 Industrielle Mikrobiologie (DE-588)4026845-7 s 3\p DE-604 Barredo, José-Luis edt Erscheint auch als Druck-Ausgabe 9781588295484 https://doi.org/10.1385/1592598471 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Microbial Processes and Products Life Sciences Microbiology Life sciences Mikroorganismus (DE-588)4039226-0 gnd Biotechnologie (DE-588)4069491-4 gnd Industrielle Mikrobiologie (DE-588)4026845-7 gnd |
subject_GND | (DE-588)4039226-0 (DE-588)4069491-4 (DE-588)4026845-7 (DE-588)4143413-4 |
title | Microbial Processes and Products |
title_auth | Microbial Processes and Products |
title_exact_search | Microbial Processes and Products |
title_full | Microbial Processes and Products edited by José-Luis Barredo |
title_fullStr | Microbial Processes and Products edited by José-Luis Barredo |
title_full_unstemmed | Microbial Processes and Products edited by José-Luis Barredo |
title_short | Microbial Processes and Products |
title_sort | microbial processes and products |
topic | Life Sciences Microbiology Life sciences Mikroorganismus (DE-588)4039226-0 gnd Biotechnologie (DE-588)4069491-4 gnd Industrielle Mikrobiologie (DE-588)4026845-7 gnd |
topic_facet | Life Sciences Microbiology Life sciences Mikroorganismus Biotechnologie Industrielle Mikrobiologie Aufsatzsammlung |
url | https://doi.org/10.1385/1592598471 |
work_keys_str_mv | AT barredojoseluis microbialprocessesandproducts |