Ion Channels: Methods and Protocols
It is now clear that ion channels play essential roles in cell biology and physiology and that their dysfunction is the root cause of many human diseases. In Ion Channels: Methods and Protocols, internationally recognized biomedical scientists describe recent technological breakthroughs and demonstr...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Schriftenreihe: | Methods in Molecular Biology™
337 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | It is now clear that ion channels play essential roles in cell biology and physiology and that their dysfunction is the root cause of many human diseases. In Ion Channels: Methods and Protocols, internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Ion Channels: Methods and Protocols offer both novice and experienced researchers a versatile collection of proven techniques for illuminating the structure, function, and regulation of ion channel proteins and finding suitable drug targets when they cause disease |
| Beschreibung: | 1 Online-Ressource (XII, 216 p. 59 illus., 1 illus. in color) |
| ISBN: | 9781597450959 |
| DOI: | 10.1385/1597450952 |
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| spelling | Ion Channels Methods and Protocols edited by James D. Stockand, Mark S. Shapiro Totowa, NJ Humana Press 2006 1 Online-Ressource (XII, 216 p. 59 illus., 1 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 337 It is now clear that ion channels play essential roles in cell biology and physiology and that their dysfunction is the root cause of many human diseases. In Ion Channels: Methods and Protocols, internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Ion Channels: Methods and Protocols offer both novice and experienced researchers a versatile collection of proven techniques for illuminating the structure, function, and regulation of ion channel proteins and finding suitable drug targets when they cause disease Life Sciences Biochemistry, general Ion Channels Life sciences Biochemistry Ionenkanal (DE-588)4138699-1 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Ionenkanal (DE-588)4138699-1 s b DE-604 Stockand, James D. edt Shapiro, Mark S. edt Erscheint auch als Druck-Ausgabe 9781588295767 https://doi.org/10.1385/1597450952 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Ion Channels Methods and Protocols Life Sciences Biochemistry, general Ion Channels Life sciences Biochemistry Ionenkanal (DE-588)4138699-1 gnd |
| subject_GND | (DE-588)4138699-1 (DE-588)4143413-4 |
| title | Ion Channels Methods and Protocols |
| title_auth | Ion Channels Methods and Protocols |
| title_exact_search | Ion Channels Methods and Protocols |
| title_full | Ion Channels Methods and Protocols edited by James D. Stockand, Mark S. Shapiro |
| title_fullStr | Ion Channels Methods and Protocols edited by James D. Stockand, Mark S. Shapiro |
| title_full_unstemmed | Ion Channels Methods and Protocols edited by James D. Stockand, Mark S. Shapiro |
| title_short | Ion Channels |
| title_sort | ion channels methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Ion Channels Life sciences Biochemistry Ionenkanal (DE-588)4138699-1 gnd |
| topic_facet | Life Sciences Biochemistry, general Ion Channels Life sciences Biochemistry Ionenkanal Aufsatzsammlung |
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