Nitric Oxide Protocols:
Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that pro...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology™
279 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects |
| Beschreibung: | 1 Online-Ressource (XII, 248 p) |
| ISBN: | 9781592598076 |
| DOI: | 10.1385/1592598072 |
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| 520 | |a Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects | ||
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| spelling | Nitric Oxide Protocols edited by Aviv Hassid Second Edition Totowa, NJ Humana Press 2004 1 Online-Ressource (XII, 248 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 279 Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects Life Sciences Biochemistry, general Life sciences Biochemistry Physiologie (DE-588)4045981-0 gnd rswk-swf Stickstoffoxide (DE-588)4057516-0 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Stickstoffoxide (DE-588)4057516-0 s Physiologie (DE-588)4045981-0 s Labortechnik (DE-588)4123602-6 s b DE-604 Hassid, Aviv edt Erscheint auch als Druck-Ausgabe 9781588292377 https://doi.org/10.1385/1592598072 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Nitric Oxide Protocols Life Sciences Biochemistry, general Life sciences Biochemistry Physiologie (DE-588)4045981-0 gnd Stickstoffoxide (DE-588)4057516-0 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4045981-0 (DE-588)4057516-0 (DE-588)4123602-6 (DE-588)4143413-4 |
| title | Nitric Oxide Protocols |
| title_auth | Nitric Oxide Protocols |
| title_exact_search | Nitric Oxide Protocols |
| title_full | Nitric Oxide Protocols edited by Aviv Hassid |
| title_fullStr | Nitric Oxide Protocols edited by Aviv Hassid |
| title_full_unstemmed | Nitric Oxide Protocols edited by Aviv Hassid |
| title_short | Nitric Oxide Protocols |
| title_sort | nitric oxide protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Physiologie (DE-588)4045981-0 gnd Stickstoffoxide (DE-588)4057516-0 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Physiologie Stickstoffoxide Labortechnik Aufsatzsammlung |
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