Drugs of Abuse: Neurological Reviews and Protocols
New molecular technologies have transformed our understanding of the neurological impact of drugs of abuse and opened many new possibilities for research. In Drugs of Abuse: Neurological Reviews and Protocols, leading scientists from all over the world review these novel research areas and provide a...
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| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2003
|
| Schriftenreihe: | Methods In Molecular Medicine™
79 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | New molecular technologies have transformed our understanding of the neurological impact of drugs of abuse and opened many new possibilities for research. In Drugs of Abuse: Neurological Reviews and Protocols, leading scientists from all over the world review these novel research areas and provide a cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Additional methods are provided for primary neural cell culture preparation, for an immunohistochemical method to examine cellular proliferation and differentiation, and for a culture method to grow viable neural progenitors. All contributors have considerable experience with the protocols covered, and each protocol has been thoroughly tried and successfully tested in the laboratory. Helpful notes for each method discuss tricks-of-the-trade, pitfalls to avoid, and troubleshooting. State-of-the-art and highly practical, Drugs of Abuse: Neurological Reviews and Protocols offers insightful reviews of key current topics and a core collection of readily reproducible techniques essential for any neurobiological laboratory carrying out either basic analyses or seeking novel research approaches |
| Beschreibung: | 1 Online-Ressource (XIV, 514 p) |
| ISBN: | 9781592593583 |
| DOI: | 10.1385/1592593585 |
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| 520 | |a New molecular technologies have transformed our understanding of the neurological impact of drugs of abuse and opened many new possibilities for research. In Drugs of Abuse: Neurological Reviews and Protocols, leading scientists from all over the world review these novel research areas and provide a cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Additional methods are provided for primary neural cell culture preparation, for an immunohistochemical method to examine cellular proliferation and differentiation, and for a culture method to grow viable neural progenitors. All contributors have considerable experience with the protocols covered, and each protocol has been thoroughly tried and successfully tested in the laboratory. Helpful notes for each method discuss tricks-of-the-trade, pitfalls to avoid, and troubleshooting. State-of-the-art and highly practical, Drugs of Abuse: Neurological Reviews and Protocols offers insightful reviews of key current topics and a core collection of readily reproducible techniques essential for any neurobiological laboratory carrying out either basic analyses or seeking novel research approaches | ||
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| spelling | Drugs of Abuse Neurological Reviews and Protocols edited by John Q. Wang Totowa, NJ Humana Press 2003 1 Online-Ressource (XIV, 514 p) txt rdacontent c rdamedia cr rdacarrier Methods In Molecular Medicine™ 79 New molecular technologies have transformed our understanding of the neurological impact of drugs of abuse and opened many new possibilities for research. In Drugs of Abuse: Neurological Reviews and Protocols, leading scientists from all over the world review these novel research areas and provide a cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Additional methods are provided for primary neural cell culture preparation, for an immunohistochemical method to examine cellular proliferation and differentiation, and for a culture method to grow viable neural progenitors. All contributors have considerable experience with the protocols covered, and each protocol has been thoroughly tried and successfully tested in the laboratory. Helpful notes for each method discuss tricks-of-the-trade, pitfalls to avoid, and troubleshooting. State-of-the-art and highly practical, Drugs of Abuse: Neurological Reviews and Protocols offers insightful reviews of key current topics and a core collection of readily reproducible techniques essential for any neurobiological laboratory carrying out either basic analyses or seeking novel research approaches Pharmacy Wang, John Q. edt Erscheint auch als Druck-Ausgabe 9781588290571 https://doi.org/10.1385/1592593585 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Drugs of Abuse Neurological Reviews and Protocols Pharmacy |
| title | Drugs of Abuse Neurological Reviews and Protocols |
| title_auth | Drugs of Abuse Neurological Reviews and Protocols |
| title_exact_search | Drugs of Abuse Neurological Reviews and Protocols |
| title_full | Drugs of Abuse Neurological Reviews and Protocols edited by John Q. Wang |
| title_fullStr | Drugs of Abuse Neurological Reviews and Protocols edited by John Q. Wang |
| title_full_unstemmed | Drugs of Abuse Neurological Reviews and Protocols edited by John Q. Wang |
| title_short | Drugs of Abuse |
| title_sort | drugs of abuse neurological reviews and protocols |
| title_sub | Neurological Reviews and Protocols |
| topic | Pharmacy |
| topic_facet | Pharmacy |
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