Hedgehog Signaling Protocols:
The second edition of this volume provides novel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and ma...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2015
|
| Ausgabe: | 2nd ed. 2015 |
| Schriftenreihe: | Methods in Molecular Biology
1322 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | The second edition of this volume provides novel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and mathematical models for studying Hh proteins gradient formation in tissues. The next few chapters explore studies in cell culture using fibroblasts that respond readily to Hh proteins. The book goes on to present protocols for evaluation of transcriptional activity of the Gli transcription factors in cell cultures and determine the expression level of endogenous Gli proteins. The final chapters talk about metabolic effects of noncanonical Hh signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Hedgehog Signaling Protocols, Second Edition presents researchers with a comprehensive understanding of the Hh pathway and its function in development and diseases |
| Beschreibung: | 1 Online-Ressource (XVI, 200 p. 42 illus., 28 illus. in color) |
| ISBN: | 9781493927722 |
| DOI: | 10.1007/978-1-4939-2772-2 |
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Datensatz im Suchindex
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| isbn | 9781493927722 |
| language | English |
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| spelling | Hedgehog Signaling Protocols edited by Natalia A. Riobo 2nd ed. 2015 New York, NY Springer New York 2015 1 Online-Ressource (XVI, 200 p. 42 illus., 28 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1322 The second edition of this volume provides novel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and mathematical models for studying Hh proteins gradient formation in tissues. The next few chapters explore studies in cell culture using fibroblasts that respond readily to Hh proteins. The book goes on to present protocols for evaluation of transcriptional activity of the Gli transcription factors in cell cultures and determine the expression level of endogenous Gli proteins. The final chapters talk about metabolic effects of noncanonical Hh signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Hedgehog Signaling Protocols, Second Edition presents researchers with a comprehensive understanding of the Hh pathway and its function in development and diseases Life Sciences Protein Science Life sciences Proteins Riobo, Natalia A. edt Erscheint auch als Druck-Ausgabe 9781493927715 https://doi.org/10.1007/978-1-4939-2772-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Hedgehog Signaling Protocols Life Sciences Protein Science Life sciences Proteins |
| title | Hedgehog Signaling Protocols |
| title_auth | Hedgehog Signaling Protocols |
| title_exact_search | Hedgehog Signaling Protocols |
| title_full | Hedgehog Signaling Protocols edited by Natalia A. Riobo |
| title_fullStr | Hedgehog Signaling Protocols edited by Natalia A. Riobo |
| title_full_unstemmed | Hedgehog Signaling Protocols edited by Natalia A. Riobo |
| title_short | Hedgehog Signaling Protocols |
| title_sort | hedgehog signaling protocols |
| topic | Life Sciences Protein Science Life sciences Proteins |
| topic_facet | Life Sciences Protein Science Life sciences Proteins |
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