Bioconjugation Protocols: Strategies and Methods
Contemporary approaches to the synthesis of chemically modified proteins not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety, but also the effective use of recent developments in microarray technologies and nanobiotechnology. In Biocon...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Schriftenreihe: | Methods in Molecular Biology™
283 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Contemporary approaches to the synthesis of chemically modified proteins not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety, but also the effective use of recent developments in microarray technologies and nanobiotechnology. In Bioconjugation Protocols: Strategies and Methods, expert laboratorians update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanoparticles, and carbon nanotube probes for atomic force microscopy. All the protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods offers both novice and experienced researchers access to the broad array of techniques needed to master the semisynthesis of functional biomolecular reagents for a wide variety of applications-ranging from novel biomedical diagnostics, to powerful new therapeutics, to advanced biomaterials |
| Beschreibung: | 1 Online-Ressource (XII, 332 p) |
| ISBN: | 9781592598137 |
| DOI: | 10.1385/1592598137 |
Internformat
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| spelling | Bioconjugation Protocols Strategies and Methods edited by Christof M. Niemeyer Totowa, NJ Humana Press 2004 1 Online-Ressource (XII, 332 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 283 Contemporary approaches to the synthesis of chemically modified proteins not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety, but also the effective use of recent developments in microarray technologies and nanobiotechnology. In Bioconjugation Protocols: Strategies and Methods, expert laboratorians update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanoparticles, and carbon nanotube probes for atomic force microscopy. All the protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods offers both novice and experienced researchers access to the broad array of techniques needed to master the semisynthesis of functional biomolecular reagents for a wide variety of applications-ranging from novel biomedical diagnostics, to powerful new therapeutics, to advanced biomaterials Life Sciences Cell Biology Life sciences Cell biology Immuncytochemie (DE-588)4161368-5 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Immuncytochemie (DE-588)4161368-5 s Labortechnik (DE-588)4123602-6 s DE-604 Niemeyer, Christof M. edt Erscheint auch als Druck-Ausgabe 9781588290984 https://doi.org/10.1385/1592598137 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Bioconjugation Protocols Strategies and Methods Life Sciences Cell Biology Life sciences Cell biology Immuncytochemie (DE-588)4161368-5 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4161368-5 (DE-588)4123602-6 (DE-588)4143413-4 |
| title | Bioconjugation Protocols Strategies and Methods |
| title_auth | Bioconjugation Protocols Strategies and Methods |
| title_exact_search | Bioconjugation Protocols Strategies and Methods |
| title_full | Bioconjugation Protocols Strategies and Methods edited by Christof M. Niemeyer |
| title_fullStr | Bioconjugation Protocols Strategies and Methods edited by Christof M. Niemeyer |
| title_full_unstemmed | Bioconjugation Protocols Strategies and Methods edited by Christof M. Niemeyer |
| title_short | Bioconjugation Protocols |
| title_sort | bioconjugation protocols strategies and methods |
| title_sub | Strategies and Methods |
| topic | Life Sciences Cell Biology Life sciences Cell biology Immuncytochemie (DE-588)4161368-5 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Immuncytochemie Labortechnik Aufsatzsammlung |
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