Therapeutic Proteins: Methods and Protocols
With the completion of the human genome, the number of protein-based drug candidates has increased dramatically, along with the need to find methods for producing these proteins on a scale large enough to meet demand without compromising the authenticity of the final product. In Therapeutic Proteins...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2005
|
| Schriftenreihe: | Methods in Molecular Biology™
308 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | With the completion of the human genome, the number of protein-based drug candidates has increased dramatically, along with the need to find methods for producing these proteins on a scale large enough to meet demand without compromising the authenticity of the final product. In Therapeutic Proteins: Methods and Protocols, world-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Therapeutic Proteins: Methods and Protocols offers both novice and experienced researchers the latest innovative, readily reproducible techniques, as well as helpful commentaries on the development and production of therapeutic proteins, ranging from their expression to actual formulation |
| Beschreibung: | 1 Online-Ressource (XIV, 482 p) |
| ISBN: | 9781592599226 |
| DOI: | 10.1385/1592599222 |
Internformat
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| 520 | |a With the completion of the human genome, the number of protein-based drug candidates has increased dramatically, along with the need to find methods for producing these proteins on a scale large enough to meet demand without compromising the authenticity of the final product. In Therapeutic Proteins: Methods and Protocols, world-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Therapeutic Proteins: Methods and Protocols offers both novice and experienced researchers the latest innovative, readily reproducible techniques, as well as helpful commentaries on the development and production of therapeutic proteins, ranging from their expression to actual formulation | ||
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| spelling | Therapeutic Proteins Methods and Protocols edited by C. Mark Smales, David C. James Totowa, NJ Humana Press 2005 1 Online-Ressource (XIV, 482 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 308 With the completion of the human genome, the number of protein-based drug candidates has increased dramatically, along with the need to find methods for producing these proteins on a scale large enough to meet demand without compromising the authenticity of the final product. In Therapeutic Proteins: Methods and Protocols, world-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Therapeutic Proteins: Methods and Protocols offers both novice and experienced researchers the latest innovative, readily reproducible techniques, as well as helpful commentaries on the development and production of therapeutic proteins, ranging from their expression to actual formulation Life Sciences Biochemistry, general Life sciences Biochemistry Proteine (DE-588)4076388-2 gnd rswk-swf Arzneimittel (DE-588)4003115-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Arzneimittel (DE-588)4003115-9 s b DE-604 Smales, C. Mark edt James, David C. edt Erscheint auch als Druck-Ausgabe 9781588293909 https://doi.org/10.1385/1592599222 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Therapeutic Proteins Methods and Protocols Life Sciences Biochemistry, general Life sciences Biochemistry Proteine (DE-588)4076388-2 gnd Arzneimittel (DE-588)4003115-9 gnd |
| subject_GND | (DE-588)4076388-2 (DE-588)4003115-9 (DE-588)4143413-4 |
| title | Therapeutic Proteins Methods and Protocols |
| title_auth | Therapeutic Proteins Methods and Protocols |
| title_exact_search | Therapeutic Proteins Methods and Protocols |
| title_full | Therapeutic Proteins Methods and Protocols edited by C. Mark Smales, David C. James |
| title_fullStr | Therapeutic Proteins Methods and Protocols edited by C. Mark Smales, David C. James |
| title_full_unstemmed | Therapeutic Proteins Methods and Protocols edited by C. Mark Smales, David C. James |
| title_short | Therapeutic Proteins |
| title_sort | therapeutic proteins methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Proteine (DE-588)4076388-2 gnd Arzneimittel (DE-588)4003115-9 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Proteine Arzneimittel Aufsatzsammlung |
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