Two-Dimensional Electrophoresis Protocols:
Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In T...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2009
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
519 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists |
| Beschreibung: | 1 Online-Ressource (XIV, 546 p. 100 illus., 1 illus. in color) |
| ISBN: | 9781597452816 |
| DOI: | 10.1007/978-1-59745-281-6 |
Internformat
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| 520 | |a Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Biochemistry, general | |
| 650 | 4 | |a Protein Science | |
| 650 | 4 | |a Proteomics | |
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| 650 | 4 | |a Biochemistry | |
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| discipline | Biologie |
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| isbn | 9781597452816 |
| language | English |
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| spelling | Two-Dimensional Electrophoresis Protocols edited by Raymond Tyther, David Sheehan Totowa, NJ Humana Press 2009 1 Online-Ressource (XIV, 546 p. 100 illus., 1 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 519 Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists Life Sciences Biochemistry, general Protein Science Proteomics Electrophoresis Life sciences Biochemistry Proteins Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Zweidimensionale Elektrophorese (DE-588)4221393-9 s Methode (DE-588)4038971-6 s 1\p DE-604 Tyther, Raymond edt Sheehan, David edt Erscheint auch als Druck-Ausgabe 9781588299376 https://doi.org/10.1007/978-1-59745-281-6 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Two-Dimensional Electrophoresis Protocols Life Sciences Biochemistry, general Protein Science Proteomics Electrophoresis Life sciences Biochemistry Proteins Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4221393-9 (DE-588)4038971-6 |
| title | Two-Dimensional Electrophoresis Protocols |
| title_auth | Two-Dimensional Electrophoresis Protocols |
| title_exact_search | Two-Dimensional Electrophoresis Protocols |
| title_full | Two-Dimensional Electrophoresis Protocols edited by Raymond Tyther, David Sheehan |
| title_fullStr | Two-Dimensional Electrophoresis Protocols edited by Raymond Tyther, David Sheehan |
| title_full_unstemmed | Two-Dimensional Electrophoresis Protocols edited by Raymond Tyther, David Sheehan |
| title_short | Two-Dimensional Electrophoresis Protocols |
| title_sort | two dimensional electrophoresis protocols |
| topic | Life Sciences Biochemistry, general Protein Science Proteomics Electrophoresis Life sciences Biochemistry Proteins Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Life Sciences Biochemistry, general Protein Science Proteomics Electrophoresis Life sciences Biochemistry Proteins Zweidimensionale Elektrophorese Methode |
| url | https://doi.org/10.1007/978-1-59745-281-6 |
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