Strain Engineering: Methods and Protocols
Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engi...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2011
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
765 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering |
| Beschreibung: | 1 Online-Ressource (XI, 480 p. 83 illus., 2 illus. in color) |
| ISBN: | 9781617791970 |
| DOI: | 10.1007/978-1-61779-197-0 |
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Datensatz im Suchindex
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| dewey-ones | 599 - Mammalia 611 - Human anatomy, cytology, histology |
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| dewey-sort | 3599.935 |
| dewey-tens | 590 - Animals 610 - Medicine and health |
| discipline | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-61779-197-0 |
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| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:36Z |
| institution | BVB |
| isbn | 9781617791970 |
| language | English |
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| physical | 1 Online-Ressource (XI, 480 p. 83 illus., 2 illus. in color) |
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| spelling | Strain Engineering Methods and Protocols edited by James A. Williams Totowa, NJ Humana Press 2011 1 Online-Ressource (XI, 480 p. 83 illus., 2 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 765 Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering Williams, James A. edt Erscheint auch als Druck-Ausgabe 9781617791963 https://doi.org/10.1007/978-1-61779-197-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Strain Engineering Methods and Protocols Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering |
| title | Strain Engineering Methods and Protocols |
| title_auth | Strain Engineering Methods and Protocols |
| title_exact_search | Strain Engineering Methods and Protocols |
| title_full | Strain Engineering Methods and Protocols edited by James A. Williams |
| title_fullStr | Strain Engineering Methods and Protocols edited by James A. Williams |
| title_full_unstemmed | Strain Engineering Methods and Protocols edited by James A. Williams |
| title_short | Strain Engineering |
| title_sort | strain engineering methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering |
| topic_facet | Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering |
| url | https://doi.org/10.1007/978-1-61779-197-0 |
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