The Genetic Manipulation of Staphylococci: Methods and Protocols
This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended chang...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2016
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| Schriftenreihe: | Methods in Molecular Biology
1373 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria |
| Beschreibung: | 1 Online-Ressource (X, 186 p. 23 illus., 7 illus. in color) |
| ISBN: | 9781493931583 |
| DOI: | 10.1007/978-1-4939-3158-3 |
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Datensatz im Suchindex
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| dewey-full | 579 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 579 - Microorganisms, fungi & algae |
| dewey-raw | 579 |
| dewey-search | 579 |
| dewey-sort | 3579 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-4939-3158-3 |
| format | Electronic eBook |
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| indexdate | 2024-07-10T08:05:36Z |
| institution | BVB |
| isbn | 9781493931583 |
| language | English |
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| physical | 1 Online-Ressource (X, 186 p. 23 illus., 7 illus. in color) |
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| spelling | The Genetic Manipulation of Staphylococci Methods and Protocols edited by Jeffrey L. Bose New York, NY Springer New York 2016 1 Online-Ressource (X, 186 p. 23 illus., 7 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1373 This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria Life Sciences Microbiology Gene Expression Life sciences Gene expression Bose, Jeffrey L. edt Erscheint auch als Druck-Ausgabe 9781493931576 https://doi.org/10.1007/978-1-4939-3158-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | The Genetic Manipulation of Staphylococci Methods and Protocols Life Sciences Microbiology Gene Expression Life sciences Gene expression |
| title | The Genetic Manipulation of Staphylococci Methods and Protocols |
| title_auth | The Genetic Manipulation of Staphylococci Methods and Protocols |
| title_exact_search | The Genetic Manipulation of Staphylococci Methods and Protocols |
| title_full | The Genetic Manipulation of Staphylococci Methods and Protocols edited by Jeffrey L. Bose |
| title_fullStr | The Genetic Manipulation of Staphylococci Methods and Protocols edited by Jeffrey L. Bose |
| title_full_unstemmed | The Genetic Manipulation of Staphylococci Methods and Protocols edited by Jeffrey L. Bose |
| title_short | The Genetic Manipulation of Staphylococci |
| title_sort | the genetic manipulation of staphylococci methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Microbiology Gene Expression Life sciences Gene expression |
| topic_facet | Life Sciences Microbiology Gene Expression Life sciences Gene expression |
| url | https://doi.org/10.1007/978-1-4939-3158-3 |
| work_keys_str_mv | AT bosejeffreyl thegeneticmanipulationofstaphylococcimethodsandprotocols |