mRNA Processing and Metabolism: Methods and Protocols
mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Schriftenreihe: | Methods in Molecular Biology™
257 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research |
| Beschreibung: | 1 Online-Ressource (XVI, 270 p) |
| ISBN: | 9781592597505 |
| DOI: | 10.1385/1592597505 |
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| spelling | mRNA Processing and Metabolism Methods and Protocols edited by Daniel R. Schoenberg Totowa, NJ Humana Press 2004 1 Online-Ressource (XVI, 270 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 257 mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research Life Sciences Cell Biology Life sciences Cell biology Messenger-RNS (DE-588)4169499-5 gnd rswk-swf Biochemische Methode (DE-588)4319880-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Messenger-RNS (DE-588)4169499-5 s Biochemische Methode (DE-588)4319880-6 s DE-604 Schoenberg, Daniel R. edt Erscheint auch als Druck-Ausgabe 9781588292254 https://doi.org/10.1385/1592597505 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | mRNA Processing and Metabolism Methods and Protocols Life Sciences Cell Biology Life sciences Cell biology Messenger-RNS (DE-588)4169499-5 gnd Biochemische Methode (DE-588)4319880-6 gnd |
| subject_GND | (DE-588)4169499-5 (DE-588)4319880-6 (DE-588)4143413-4 |
| title | mRNA Processing and Metabolism Methods and Protocols |
| title_auth | mRNA Processing and Metabolism Methods and Protocols |
| title_exact_search | mRNA Processing and Metabolism Methods and Protocols |
| title_full | mRNA Processing and Metabolism Methods and Protocols edited by Daniel R. Schoenberg |
| title_fullStr | mRNA Processing and Metabolism Methods and Protocols edited by Daniel R. Schoenberg |
| title_full_unstemmed | mRNA Processing and Metabolism Methods and Protocols edited by Daniel R. Schoenberg |
| title_short | mRNA Processing and Metabolism |
| title_sort | mrna processing and metabolism methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Messenger-RNS (DE-588)4169499-5 gnd Biochemische Methode (DE-588)4319880-6 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Messenger-RNS Biochemische Methode Aufsatzsammlung |
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