Transgenesis Techniques: Principles and Protocols
The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to prese...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Springer New York
2002
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology
180 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes |
| Beschreibung: | 1 Online-Ressource (X, 351 p) |
| ISBN: | 9781592591787 |
| DOI: | 10.1385/1592591787 |
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Datensatz im Suchindex
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| spelling | Transgenesis Techniques Principles and Protocols edited by Alan R. Clarke Second Edition Totowa, NJ Springer New York 2002 1 Online-Ressource (X, 351 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 180 The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes Life Sciences Cell Biology Life sciences Cell biology Gentransfer (DE-588)4194102-0 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Transgene Tiere (DE-588)4279021-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Transgene Tiere (DE-588)4279021-9 s Methode (DE-588)4038971-6 s DE-604 Polymerase-Kettenreaktion (DE-588)4256726-9 s Gentransfer (DE-588)4194102-0 s Clarke, Alan R. edt Erscheint auch als Druck-Ausgabe 9780896036963 https://doi.org/10.1385/1592591787 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Transgenesis Techniques Principles and Protocols Life Sciences Cell Biology Life sciences Cell biology Gentransfer (DE-588)4194102-0 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd Transgene Tiere (DE-588)4279021-9 gnd |
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| title | Transgenesis Techniques Principles and Protocols |
| title_auth | Transgenesis Techniques Principles and Protocols |
| title_exact_search | Transgenesis Techniques Principles and Protocols |
| title_full | Transgenesis Techniques Principles and Protocols edited by Alan R. Clarke |
| title_fullStr | Transgenesis Techniques Principles and Protocols edited by Alan R. Clarke |
| title_full_unstemmed | Transgenesis Techniques Principles and Protocols edited by Alan R. Clarke |
| title_short | Transgenesis Techniques |
| title_sort | transgenesis techniques principles and protocols |
| title_sub | Principles and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Gentransfer (DE-588)4194102-0 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd Transgene Tiere (DE-588)4279021-9 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Gentransfer Polymerase-Kettenreaktion Methode Transgene Tiere Aufsatzsammlung |
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