In Situ Hybridization Protocols:
In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniqu...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Ausgabe: | Third Edition |
| Schriftenreihe: | Methods in Molecular Biology™
326 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully |
| Beschreibung: | 1 Online-Ressource (XII, 270 p. 68 illus., 1 illus. in color) |
| ISBN: | 9781597450072 |
| DOI: | 10.1385/1597450073 |
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| 520 | |a In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully | ||
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| spelling | In Situ Hybridization Protocols edited by Ian A. Darby, Tim D. Hewitson Third Edition Totowa, NJ Humana Press 2006 1 Online-Ressource (XII, 270 p. 68 illus., 1 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 326 In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology In-situ-Hybridisierung (DE-588)4215968-4 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content In-situ-Hybridisierung (DE-588)4215968-4 s DE-604 Darby, Ian A. edt Hewitson, Tim D. edt Erscheint auch als Druck-Ausgabe 9781588294029 https://doi.org/10.1385/1597450073 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | In Situ Hybridization Protocols Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology In-situ-Hybridisierung (DE-588)4215968-4 gnd |
| subject_GND | (DE-588)4215968-4 (DE-588)4143413-4 |
| title | In Situ Hybridization Protocols |
| title_auth | In Situ Hybridization Protocols |
| title_exact_search | In Situ Hybridization Protocols |
| title_full | In Situ Hybridization Protocols edited by Ian A. Darby, Tim D. Hewitson |
| title_fullStr | In Situ Hybridization Protocols edited by Ian A. Darby, Tim D. Hewitson |
| title_full_unstemmed | In Situ Hybridization Protocols edited by Ian A. Darby, Tim D. Hewitson |
| title_short | In Situ Hybridization Protocols |
| title_sort | in situ hybridization protocols |
| topic | Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology In-situ-Hybridisierung (DE-588)4215968-4 gnd |
| topic_facet | Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology In-situ-Hybridisierung Aufsatzsammlung |
| url | https://doi.org/10.1385/1597450073 |
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