Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology
The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access, at the ear- est stages, to the genomic ba...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1997
|
| Schriftenreihe: | Neuromethods
28 |
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access, at the ear- est stages, to the genomic basis underlying the system’s ultimate complexity, as exhibited once its structures are fully formed. Yet, nerve cells are anatomically, physiolo- cally, and biochemically diverse. The multitude of d- tinctly different routes for their development thus makes the developing nervous system especially intriguing for molecular neurobiologists. In particular, the demands of modern molecular neuroscience call for the establishment of efficient yet versatile systems for studying these c- plex processes. Transgenic embryos of the frog Xenopus laevis offer an excellent system for approaching neuroscientific issues. Insertion of foreign genes is performed simply, by mic- injection under binocular observation; hundreds of in vitro-fertilized embryos can be microinjected in one experiment. Embryos develop in tap water, at room t- perature, and within a few days become independent swimming tadpoles with fully functioning neuromus- lar systems. Being relatively small, these organisms are amenable to detailed analyses at the levels of mRNA, protein, and cell. Their rapid development permits the study of morphogenetic processes involved in early development, such as myogenesis and neural induction, as well as those involved in organogenesis and formation of the brain, the musculature, and the interconnections between them. Foreign DNA remains predominantly extrachromosomal |
| Beschreibung: | 1 Online-Ressource (XVIII, 198 p) |
| ISBN: | 9781592596331 |
| DOI: | 10.1385/0896034577 |
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Datensatz im Suchindex
| _version_ | 1804178540462080000 |
|---|---|
| any_adam_object | |
| author | Seidman, Shlomo Soreq, Hermona |
| author_facet | Seidman, Shlomo Soreq, Hermona |
| author_role | aut aut |
| author_sort | Seidman, Shlomo |
| author_variant | s s ss h s hs |
| building | Verbundindex |
| bvnumber | BV044951617 |
| classification_rvk | WW 2200 |
| collection | ZDB-2-PRO |
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| dewey-full | 612.8 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 612 - Human physiology |
| dewey-raw | 612.8 |
| dewey-search | 612.8 |
| dewey-sort | 3612.8 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| doi_str_mv | 10.1385/0896034577 |
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| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:36Z |
| institution | BVB |
| isbn | 9781592596331 |
| language | English |
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| spelling | Seidman, Shlomo Verfasser aut Transgenic Xenopus Microinjection Methods and Developmental Neurobiology by Shlomo Seidman, Hermona Soreq Totowa, NJ Humana Press 1997 1 Online-Ressource (XVIII, 198 p) txt rdacontent c rdamedia cr rdacarrier Neuromethods 28 The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access, at the ear- est stages, to the genomic basis underlying the system’s ultimate complexity, as exhibited once its structures are fully formed. Yet, nerve cells are anatomically, physiolo- cally, and biochemically diverse. The multitude of d- tinctly different routes for their development thus makes the developing nervous system especially intriguing for molecular neurobiologists. In particular, the demands of modern molecular neuroscience call for the establishment of efficient yet versatile systems for studying these c- plex processes. Transgenic embryos of the frog Xenopus laevis offer an excellent system for approaching neuroscientific issues. Insertion of foreign genes is performed simply, by mic- injection under binocular observation; hundreds of in vitro-fertilized embryos can be microinjected in one experiment. Embryos develop in tap water, at room t- perature, and within a few days become independent swimming tadpoles with fully functioning neuromus- lar systems. Being relatively small, these organisms are amenable to detailed analyses at the levels of mRNA, protein, and cell. Their rapid development permits the study of morphogenetic processes involved in early development, such as myogenesis and neural induction, as well as those involved in organogenesis and formation of the brain, the musculature, and the interconnections between them. Foreign DNA remains predominantly extrachromosomal Biomedicine Neurosciences Medicine Neurobiologie (DE-588)4041871-6 gnd rswk-swf Mikroinjektion (DE-588)4404677-7 gnd rswk-swf Entwicklungsbiologie (DE-588)4152440-8 gnd rswk-swf Krallenfrosch (DE-588)4165479-1 gnd rswk-swf Transgene Tiere (DE-588)4279021-9 gnd rswk-swf Krallenfrosch (DE-588)4165479-1 s Transgene Tiere (DE-588)4279021-9 s DE-604 Mikroinjektion (DE-588)4404677-7 s Entwicklungsbiologie (DE-588)4152440-8 s Neurobiologie (DE-588)4041871-6 s Soreq, Hermona aut Erscheint auch als Druck-Ausgabe 9780896034570 https://doi.org/10.1385/0896034577 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Seidman, Shlomo Soreq, Hermona Transgenic Xenopus Microinjection Methods and Developmental Neurobiology Biomedicine Neurosciences Medicine Neurobiologie (DE-588)4041871-6 gnd Mikroinjektion (DE-588)4404677-7 gnd Entwicklungsbiologie (DE-588)4152440-8 gnd Krallenfrosch (DE-588)4165479-1 gnd Transgene Tiere (DE-588)4279021-9 gnd |
| subject_GND | (DE-588)4041871-6 (DE-588)4404677-7 (DE-588)4152440-8 (DE-588)4165479-1 (DE-588)4279021-9 |
| title | Transgenic Xenopus Microinjection Methods and Developmental Neurobiology |
| title_auth | Transgenic Xenopus Microinjection Methods and Developmental Neurobiology |
| title_exact_search | Transgenic Xenopus Microinjection Methods and Developmental Neurobiology |
| title_full | Transgenic Xenopus Microinjection Methods and Developmental Neurobiology by Shlomo Seidman, Hermona Soreq |
| title_fullStr | Transgenic Xenopus Microinjection Methods and Developmental Neurobiology by Shlomo Seidman, Hermona Soreq |
| title_full_unstemmed | Transgenic Xenopus Microinjection Methods and Developmental Neurobiology by Shlomo Seidman, Hermona Soreq |
| title_short | Transgenic Xenopus |
| title_sort | transgenic xenopus microinjection methods and developmental neurobiology |
| title_sub | Microinjection Methods and Developmental Neurobiology |
| topic | Biomedicine Neurosciences Medicine Neurobiologie (DE-588)4041871-6 gnd Mikroinjektion (DE-588)4404677-7 gnd Entwicklungsbiologie (DE-588)4152440-8 gnd Krallenfrosch (DE-588)4165479-1 gnd Transgene Tiere (DE-588)4279021-9 gnd |
| topic_facet | Biomedicine Neurosciences Medicine Neurobiologie Mikroinjektion Entwicklungsbiologie Krallenfrosch Transgene Tiere |
| url | https://doi.org/10.1385/0896034577 |
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