In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols
The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-a...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2012
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| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
872 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies |
| Beschreibung: | 1 Online-Ressource (XIII, 269 p. 70 illus., 64 illus. in color) |
| ISBN: | 9781617797972 |
| DOI: | 10.1007/978-1-61779-797-2 |
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| spelling | In Vivo Cellular Imaging Using Fluorescent Proteins Methods and Protocols edited by Robert M. Hoffman Totowa, NJ Humana Press 2012 1 Online-Ressource (XIII, 269 p. 70 illus., 64 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 872 The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies Medicine & Public Health Imaging / Radiology Medicine Radiology Cytologie (DE-588)4070177-3 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Fluoreszenzmikroskopie (DE-588)4290958-2 gnd rswk-swf Cytologie (DE-588)4070177-3 s Fluoreszenzmikroskopie (DE-588)4290958-2 s Labortechnik (DE-588)4123602-6 s b DE-604 Hoffman, Robert M. edt Erscheint auch als Druck-Ausgabe 9781617797965 https://doi.org/10.1007/978-1-61779-797-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | In Vivo Cellular Imaging Using Fluorescent Proteins Methods and Protocols Medicine & Public Health Imaging / Radiology Medicine Radiology Cytologie (DE-588)4070177-3 gnd Labortechnik (DE-588)4123602-6 gnd Fluoreszenzmikroskopie (DE-588)4290958-2 gnd |
| subject_GND | (DE-588)4070177-3 (DE-588)4123602-6 (DE-588)4290958-2 |
| title | In Vivo Cellular Imaging Using Fluorescent Proteins Methods and Protocols |
| title_auth | In Vivo Cellular Imaging Using Fluorescent Proteins Methods and Protocols |
| title_exact_search | In Vivo Cellular Imaging Using Fluorescent Proteins Methods and Protocols |
| title_full | In Vivo Cellular Imaging Using Fluorescent Proteins Methods and Protocols edited by Robert M. Hoffman |
| title_fullStr | In Vivo Cellular Imaging Using Fluorescent Proteins Methods and Protocols edited by Robert M. Hoffman |
| title_full_unstemmed | In Vivo Cellular Imaging Using Fluorescent Proteins Methods and Protocols edited by Robert M. Hoffman |
| title_short | In Vivo Cellular Imaging Using Fluorescent Proteins |
| title_sort | in vivo cellular imaging using fluorescent proteins methods and protocols |
| title_sub | Methods and Protocols |
| topic | Medicine & Public Health Imaging / Radiology Medicine Radiology Cytologie (DE-588)4070177-3 gnd Labortechnik (DE-588)4123602-6 gnd Fluoreszenzmikroskopie (DE-588)4290958-2 gnd |
| topic_facet | Medicine & Public Health Imaging / Radiology Medicine Radiology Cytologie Labortechnik Fluoreszenzmikroskopie |
| url | https://doi.org/10.1007/978-1-61779-797-2 |
| work_keys_str_mv | AT hoffmanrobertm invivocellularimagingusingfluorescentproteinsmethodsandprotocols |