Lentivirus Gene Engineering Protocols:
Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assem...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2003
|
| Schriftenreihe: | Methods in Molecular Biology™
229 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit. Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS. |
| Beschreibung: | 1 Online-Ressource (XII, 316 p) |
| ISBN: | 9781592593934 |
| DOI: | 10.1385/1592593933 |
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| spelling | Lentivirus Gene Engineering Protocols edited by Maurizio Federico Totowa, NJ Humana Press 2003 1 Online-Ressource (XII, 316 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 229 Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit. Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS. Life Sciences Cell Biology Life sciences Cell biology Gentransfer (DE-588)4194102-0 gnd rswk-swf Lentiviren (DE-588)4276396-4 gnd rswk-swf Gentherapie (DE-588)4296957-8 gnd rswk-swf Vektor Genetik (DE-588)4279246-0 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Gentransfer (DE-588)4194102-0 s Lentiviren (DE-588)4276396-4 s Vektor Genetik (DE-588)4279246-0 s DE-604 Gentherapie (DE-588)4296957-8 s Federico, Maurizio edt Erscheint auch als Druck-Ausgabe 9781588290915 https://doi.org/10.1385/1592593933 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Lentivirus Gene Engineering Protocols Life Sciences Cell Biology Life sciences Cell biology Gentransfer (DE-588)4194102-0 gnd Lentiviren (DE-588)4276396-4 gnd Gentherapie (DE-588)4296957-8 gnd Vektor Genetik (DE-588)4279246-0 gnd |
| subject_GND | (DE-588)4194102-0 (DE-588)4276396-4 (DE-588)4296957-8 (DE-588)4279246-0 (DE-588)4143413-4 |
| title | Lentivirus Gene Engineering Protocols |
| title_auth | Lentivirus Gene Engineering Protocols |
| title_exact_search | Lentivirus Gene Engineering Protocols |
| title_full | Lentivirus Gene Engineering Protocols edited by Maurizio Federico |
| title_fullStr | Lentivirus Gene Engineering Protocols edited by Maurizio Federico |
| title_full_unstemmed | Lentivirus Gene Engineering Protocols edited by Maurizio Federico |
| title_short | Lentivirus Gene Engineering Protocols |
| title_sort | lentivirus gene engineering protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Gentransfer (DE-588)4194102-0 gnd Lentiviren (DE-588)4276396-4 gnd Gentherapie (DE-588)4296957-8 gnd Vektor Genetik (DE-588)4279246-0 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Gentransfer Lentiviren Gentherapie Vektor Genetik Aufsatzsammlung |
| url | https://doi.org/10.1385/1592593933 |
| work_keys_str_mv | AT federicomaurizio lentivirusgeneengineeringprotocols |