Bacterial Transcriptional Control: Methods and Protocols
This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stabi...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2015
|
| Schriftenreihe: | Methods in Molecular Biology
1276 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future |
| Beschreibung: | 1 Online-Ressource (XI, 342 p. 48 illus., 25 illus. in color) |
| ISBN: | 9781493923922 |
| DOI: | 10.1007/978-1-4939-2392-2 |
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Datensatz im Suchindex
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| dewey-full | 579 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 579 - Microorganisms, fungi & algae |
| dewey-raw | 579 |
| dewey-search | 579 |
| dewey-sort | 3579 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-4939-2392-2 |
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| language | English |
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| spelling | Bacterial Transcriptional Control Methods and Protocols edited by Irina Artsimovitch, Thomas J. Santangelo New York, NY Springer New York 2015 1 Online-Ressource (XI, 342 p. 48 illus., 25 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1276 This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future Life Sciences Microbiology Bacteriology Genetic Engineering Life sciences Genetic engineering Artsimovitch, Irina edt Santangelo, Thomas J. edt Erscheint auch als Druck-Ausgabe 9781493923915 https://doi.org/10.1007/978-1-4939-2392-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Bacterial Transcriptional Control Methods and Protocols Life Sciences Microbiology Bacteriology Genetic Engineering Life sciences Genetic engineering |
| title | Bacterial Transcriptional Control Methods and Protocols |
| title_auth | Bacterial Transcriptional Control Methods and Protocols |
| title_exact_search | Bacterial Transcriptional Control Methods and Protocols |
| title_full | Bacterial Transcriptional Control Methods and Protocols edited by Irina Artsimovitch, Thomas J. Santangelo |
| title_fullStr | Bacterial Transcriptional Control Methods and Protocols edited by Irina Artsimovitch, Thomas J. Santangelo |
| title_full_unstemmed | Bacterial Transcriptional Control Methods and Protocols edited by Irina Artsimovitch, Thomas J. Santangelo |
| title_short | Bacterial Transcriptional Control |
| title_sort | bacterial transcriptional control methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Microbiology Bacteriology Genetic Engineering Life sciences Genetic engineering |
| topic_facet | Life Sciences Microbiology Bacteriology Genetic Engineering Life sciences Genetic engineering |
| url | https://doi.org/10.1007/978-1-4939-2392-2 |
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