In Vitro Mutagenesis: Methods and Protocols
In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis,...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
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| Schriftenreihe: | Methods in Molecular Biology
1498 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering |
| Beschreibung: | 1 Online-Ressource (XV, 511 p. 96 illus., 52 illus. in color) |
| ISBN: | 9781493964727 |
| DOI: | 10.1007/978-1-4939-6472-7 |
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| isbn | 9781493964727 |
| language | English |
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| spelling | In Vitro Mutagenesis Methods and Protocols edited by Andrew Reeves New York, NY Springer New York 2017 1 Online-Ressource (XV, 511 p. 96 illus., 52 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1498 In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering Biomedicine Human Genetics Medicine Human genetics Reeves, Andrew edt Erscheint auch als Druck-Ausgabe 9781493964703 https://doi.org/10.1007/978-1-4939-6472-7 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | In Vitro Mutagenesis Methods and Protocols Biomedicine Human Genetics Medicine Human genetics |
| title | In Vitro Mutagenesis Methods and Protocols |
| title_auth | In Vitro Mutagenesis Methods and Protocols |
| title_exact_search | In Vitro Mutagenesis Methods and Protocols |
| title_full | In Vitro Mutagenesis Methods and Protocols edited by Andrew Reeves |
| title_fullStr | In Vitro Mutagenesis Methods and Protocols edited by Andrew Reeves |
| title_full_unstemmed | In Vitro Mutagenesis Methods and Protocols edited by Andrew Reeves |
| title_short | In Vitro Mutagenesis |
| title_sort | in vitro mutagenesis methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics |
| topic_facet | Biomedicine Human Genetics Medicine Human genetics |
| url | https://doi.org/10.1007/978-1-4939-6472-7 |
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