Signal Transduction Immunohistochemistry: Methods and Protocols
Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addres...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2011
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| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
717 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints |
| Beschreibung: | 1 Online-Ressource (XIII, 302 p) |
| ISBN: | 9781617790249 |
| DOI: | 10.1007/978-1-61779-024-9 |
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| 520 | |a Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints | ||
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| spelling | Signal Transduction Immunohistochemistry Methods and Protocols edited by Alexander E. Kalyuzhny Totowa, NJ Humana Press 2011 1 Online-Ressource (XIII, 302 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 717 Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints Life Sciences Biochemistry, general Antibodies Life sciences Biochemistry Kalyuzhny, Alexander E. edt Erscheint auch als Druck-Ausgabe 9781617790232 https://doi.org/10.1007/978-1-61779-024-9 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Signal Transduction Immunohistochemistry Methods and Protocols Life Sciences Biochemistry, general Antibodies Life sciences Biochemistry |
| title | Signal Transduction Immunohistochemistry Methods and Protocols |
| title_auth | Signal Transduction Immunohistochemistry Methods and Protocols |
| title_exact_search | Signal Transduction Immunohistochemistry Methods and Protocols |
| title_full | Signal Transduction Immunohistochemistry Methods and Protocols edited by Alexander E. Kalyuzhny |
| title_fullStr | Signal Transduction Immunohistochemistry Methods and Protocols edited by Alexander E. Kalyuzhny |
| title_full_unstemmed | Signal Transduction Immunohistochemistry Methods and Protocols edited by Alexander E. Kalyuzhny |
| title_short | Signal Transduction Immunohistochemistry |
| title_sort | signal transduction immunohistochemistry methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Antibodies Life sciences Biochemistry |
| topic_facet | Life Sciences Biochemistry, general Antibodies Life sciences Biochemistry |
| url | https://doi.org/10.1007/978-1-61779-024-9 |
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